ArticleJournal of visualized experiments : JoVE2025
Assessment of Knee Hyperalgesia in Mice using Pressure Application Measurement.
Article in Journal of visualized experiments : JoVE, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Assessment of knee hyperalgesia provides a robust assay for measuring peripheral sensitization in the knee, and represents a clinically relevant pain-dependent behavior that can be used in rodent models of arthritis. Several methods have been reported to determine the threshold for responses to knee compression in rodents. Here, we aimed to adapt existing methods to develop a standardized method for assessing knee hyperalgesia in adult mice, using a Pressure Application Measurement (PAM) device. The protocol includes detailed steps, training recommendations for new experimenters, and tips for achieving consistent results. We provide suggestions for optimal training of a new experimenter. Finally, representative outcomes are provided for both acute models of transient hyperalgesia and experimental osteoarthritis (OA) models, which are characterized by chronic pain. This method is highly sensitive to pharmacological interventions, making it suitable for evaluating the effects of mediators like cytokines and for medium-throughput drug screening assays. In summary, the described approach provides a robust and clinically relevant method for quantifying knee hyperalgesia.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.