Evidence map›Paper›PMID 40586935›Full record

ArticleApplied microbiology and biotechnology2025

Engineering Sanghuangporus sanghuang for enhanced (-)-aristolone production via metabolic pathway optimization and terpene synthase engineering.

Yihan Li, Chuanzhi Kang, Jiahui Xu, Wenqing Zhou, Weishan Pan, Daofang Xia, Jian Liang, Lanping Guo, Xiao-Kui Ma

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yihan LiKey Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, College of Life Science, Ministry of Education, Shaanxi Normal University, Xi'an, 710055, Shaanxi, China.
Chuanzhi KangState Key Laboratory for Quality Ensurance and Sustainable Use of Dao-Di Herbs, National Resource Center for Chinese Materia Medical, China Academy of Chinese Medical Sciences, Beijing, 100700, People's Republic of China.
Jiahui XuKey Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, College of Life Science, Ministry of Education, Shaanxi Normal University, Xi'an, 710055, Shaanxi, China.
Wenqing ZhouInstitute of Plant Protection, Ningxia Academy of Agricultural and Forestry Sciences, Yinchuan, 750002, People's Republic of China.
Weishan PanKey Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, College of Life Science, Ministry of Education, Shaanxi Normal University, Xi'an, 710055, Shaanxi, China.
Daofang XiaInstitute of Plant Protection, Ningxia Academy of Agricultural and Forestry Sciences, Yinchuan, 750002, People's Republic of China.
Jian LiangKey Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, College of Life Science, Ministry of Education, Shaanxi Normal University, Xi'an, 710055, Shaanxi, China.
Lanping GuoState Key Laboratory for Quality Ensurance and Sustainable Use of Dao-Di Herbs, National Resource Center for Chinese Materia Medical, China Academy of Chinese Medical Sciences, Beijing, 100700, People's Republic of China. glp01@126.com.
Xiao-Kui MaKey Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, College of Life Science, Ministry of Education, Shaanxi Normal University, Xi'an, 710055, Shaanxi, China. bioma2003@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

(-)-Aristolone, a sesquiterpene with promising therapeutic properties such as antidiabetic and vasorelaxant effects, currently suffers from limited availability due to inefficient chemical synthesis and lack of viable extraction methods. This study presents a novel strategy for high-yield microbial (-)-aristolone production using Sanghuangporus sanghuang DM989 as a fungal chassis. Genome mining identified nine sesquiterpene synthases, among which TPS2152 was functionally linked to (-)-aristolone biosynthesis. TPS2152 harbors a rare DQxxD motif, diverging from the canonical DDxxD motif in plants, suggesting unique catalytic properties in fungi. Overexpression of farnesyl pyrophosphate synthase (FPPS) increased FPP precursor supply, resulting in a 78.79% rise in squalene content (1.18 mg/g) and enabling de novo (-)-aristolone synthesis (0.42 mg/g) in the FPPS

Indexed as

Alkyl and Aryl TransferasesMetabolic EngineeringMetabolic Networks and PathwaysGeranyltranstransferaseSesquiterpenesSqualeneAlkyl and Aryl TransferasesGeranyltranstransferaseSesquiterpenesSqualeneterpene synthase(-)-AristoloneFermentation kineticsSanghuangporus sanghuangSesquiterpene synthaseSesquiterpenoidsTerpenoid synthesis (TPS)

Identifiers

PMID40586935
PMCPMC12209383

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.