Evidence map›Paper›PMID 40586861›Full record

ArticleAngewandte Chemie (International ed. in English)2025

Developing HaloTag and SNAP-Tag Chemical Inducers of Dimerization to Probe Receptor Oligomerization and Downstream Signaling.

Michael Trumpp, Blaise Gatin-Fraudet, Kjell Bruckmann, Wiktor Burdzinski, Kilian Roßmann, Joshua Levitz, Petra Knaus, Jerome Jatzlau, Johannes Broichhagen

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In one paragraph

Article in Angewandte Chemie (International ed. in English), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Michael TrumppInstitute of Chemistry and Biochemistry - Biochemistry, Freie Universität Berlin, Berlin, Germany.ORCID 0000-0002-6151-9151
Blaise Gatin-FraudetLeibniz-Forschungsinstitut für Molekulare Pharmakologie, Berlin, Germany.ORCID 0000-0002-9000-9820
Kjell BruckmannInstitute of Chemistry and Biochemistry - Biochemistry, Freie Universität Berlin, Berlin, Germany.ORCID 0009-0008-0252-7048
Wiktor BurdzinskiInstitute of Chemistry and Biochemistry - Biochemistry, Freie Universität Berlin, Berlin, Germany.ORCID 0000-0002-4495-5779
Kilian RoßmannLeibniz-Forschungsinstitut für Molekulare Pharmakologie, Berlin, Germany.ORCID 0000-0003-4965-9669
Joshua LevitzDepartment of Biochemistry, Weill Cornell Medicine, New York, NY, USA.ORCID 0000-0002-8169-6323
Petra KnausInstitute of Chemistry and Biochemistry - Biochemistry, Freie Universität Berlin, Berlin, Germany.
Jerome JatzlauInstitute of Chemistry and Biochemistry - Biochemistry, Freie Universität Berlin, Berlin, Germany.ORCID 0000-0002-1757-0267
Johannes BroichhagenLeibniz-Forschungsinstitut für Molekulare Pharmakologie, Berlin, Germany.ORCID 0000-0003-3084-6595

Funding

Einstein Center for Regenerative TherapiesEuropean Union's Horizon Europe Framework Programme 101042046German Research Foundation SFB 1444IPSEN GmbHMSCA Postdoctoral Fellowship 101147198
6 · The paper itself

Abstract

Controlling protein-protein interactions is critical for dissecting signaling pathways, especially those initiated by ligand-receptor interactions, which alter receptor oligomerization and drive downstream signaling cascades. Traditional methods for driving protein-protein complexes use antibodies that face limitations in terms of stoichiometry, geometric rigidity, and antibody specificity. Chemical inducers of dimerization (CIDs) for fusion proteins such as HaloTag (Halo) and SNAP-Tags (SNAP) offer precise and covalent control of protein proximities, overcoming limitations of antibody-dependent methods. In this study, we expand the toolkit of Halo and SNAP CIDs with (1) benzylguanine (BG) and HaloTag ligand (HTL) crosslinkers featuring varying polyethylene glycol linker lengths and update this kit with (2) a FRET-based dimerizing sensor to induce and verify protein proximity. Here we establish our CIDs on extracellularly Halo- and SNAP-tagged TGFβ, BMP, neurotrophic factor, and metabotropic glutamate receptors, thereby elucidating the signaling potential of ligand-independent dimerization in a heteromeric fashion.

Indexed as

Receptors, Metabotropic GlutamateSignal TransductionDimerizationFluorescence Resonance Energy TransferGuanineHumansLigandsProtein MultimerizationGuanineLigandsReceptors, Metabotropic GlutamateBMP/TGFβ and TrkB signalingChemical inducers of dimerizationFRET sensorHaloTagProtein proximityReceptor oligomerizationSNAP‐Tag

Identifiers

PMID40586861
PMCPMC12377427

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.