Evidence map›Paper›PMID 40586712›Full record

ArticleThe Journal of cell biology2025

The PAX3-FOXO1 fusion gene reduces cell-ECM interactions and TGFβ signaling in rhabdomyosarcoma.

Antonios Chronopoulos, Ivan Chavez, Chandra Kaladhar Vemula, Nikhil Mittal, Vic Zamloot, Yuanzhong Pan, Sangyoon J Han, JinSeok Park

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of cell biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Antonios Chronopoulos *Cancer and Blood Disease Institute, Children's Hospital Los Angeles , Los Angeles, CA, USA.ORCID 0009-0006-1197-2341
Ivan Chavez *Cancer and Blood Disease Institute, Children's Hospital Los Angeles , Los Angeles, CA, USA.ORCID 0009-0000-6923-7135
Chandra Kaladhar Vemula *Cancer and Blood Disease Institute, Children's Hospital Los Angeles , Los Angeles, CA, USA.ORCID 0000-0001-6714-2829
Nikhil MittalDepartment of Biomedical Engineering, Michigan Technological University, Houghton, MI, USA.ORCID 0000-0001-8499-7749
Vic ZamlootCancer and Blood Disease Institute, Children's Hospital Los Angeles , Los Angeles, CA, USA.ORCID 0009-0005-5085-5634
Yuanzhong PanCancer and Blood Disease Institute, Children's Hospital Los Angeles , Los Angeles, CA, USA.ORCID 0000-0002-6642-758X
Sangyoon J HanDepartment of Biomedical Engineering, Michigan Technological University, Houghton, MI, USA.ORCID 0000-0002-1384-665X
JinSeok ParkCancer and Blood Disease Institute, Children's Hospital Los Angeles , Los Angeles, CA, USA.ORCID 0000-0001-6930-895X

Funding

Nascent Adhesion-Based Mechano-transmission for Extracellular Matrix Stiffness SensingR15GM135806 · NIGMS · MICHIGAN TECHNOLOGICAL UNIVERSITY · PI HAN, SANGYOON JOSHUA · 2019 to 2024
$964k
Children's Hospital Los AngelesConcern FoundationKure It Cancer ResearchNIGMS NIH HHS R15 GM135806NIH HHS 2R15GM135806-02The Saban Research Institute Intramural
6 · The paper itself

Abstract

We identify downregulation of genes related to cell-ECM interactions and TGFβ signaling in FPRMS. We confirm that TGFβ signaling enhances cell-ECM interactions in FNRMS, utilizing confocal reflection microscopy to assess ECM remodeling, and a live-cell sensor to quantitatively assess TGFβ signaling. We also show that PAX3-FOXO1 increases NOS1 expression, stimulating nitric oxide synthesis, which inhibits TGFβ signaling and reduces cell-ECM interactions. We suggest that PAX3-FOXO1 reprograms ECM anchorage dependence by suppressing cell-ECM interactions. The fusion gene can determine sensitivity to growth inhibition via targeted disruption of cell-ECM interactions or TGFβ signaling. Reduced anchorage reliance by the gene may allow cells to survive in circulation and enhance FPRMS metastatic potential.

Indexed as

Extracellular MatrixOncogene Proteins, FusionPaired Box Transcription FactorsRhabdomyosarcomaTransforming Growth Factor betaCell Line, TumorForkhead Box Protein O1Gene Expression Regulation, NeoplasticHumansNitric Oxide Synthase Type ISignal TransductionForkhead Box Protein O1Nitric Oxide Synthase Type INOS1 protein, humanOncogene Proteins, FusionPaired Box Transcription FactorsPAX3-FOXO1A fusion protein, humanTransforming Growth Factor beta

Identifiers

PMID40586712
PMCPMC12207912

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.