Evidence map›Paper›PMID 40586282›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025

Tracing and Capturing the Epiblast Pluripotency of Sheep Preimplantation Embryos.

Jinying Zhang, Runbo Li, Ruijie Luo, Qiang Zhang, Zimo Zhao, Haishen Xu, Minglei Zhi, Wenjie Jiang, Meng Wang, Xinze Chen and 19 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Tracing and Capturing the Epiblast Pluripotency of Sheep Preimplantation Embryos.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

29 authors.

Jinying ZhangState Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
Runbo LiState Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
Ruijie LuoAnimal Science and Technology College, Beijing University of Agriculture, Beijing, 102206, China.
Qiang ZhangInstitute of Cardiovascular Diseases, Xiamen Cardiovascular Hospital, School of Medicine, Xiamen University, Xiamen, 361006, China.
Zimo ZhaoState Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
Haishen XuState Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
Minglei ZhiState Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
Wenjie JiangCollege of Animal Science and Technology, Northeast Agricultural University, Harbin, 150038, China.
Meng WangState Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
Xinze ChenState Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
Zhiqiang FengState Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
Yingjie WangState Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
Yuhan YangState Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
Pengcheng HeState Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
Hanyue SuAnimal Science and Technology College, Beijing University of Agriculture, Beijing, 102206, China.
Tianzhi ChenState Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
Shunxin WangState Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
Yixuan YaoState Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
Jinghui YangAnimal Science and Technology College, Beijing University of Agriculture, Beijing, 102206, China.
Fan ZhaoAnimal Science and Technology College, Beijing University of Agriculture, Beijing, 102206, China.
Tingting LiState Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
Haitao WangState Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
Xiaosheng ZhangInstitute of Animal Sciences and Veterinary, Tianjin Academy of Agriculture Sciences, Tianjin, 300380, China.
Jinlong ZhangInstitute of Animal Sciences and Veterinary, Tianjin Academy of Agriculture Sciences, Tianjin, 300380, China.
Qiuyue LiuState Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
Shuai GaoState Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
Yuchang YaoCollege of Animal Science and Technology, Northeast Agricultural University, Harbin, 150038, China.
Jianyong HanState Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
Suying CaoAnimal Science and Technology College, Beijing University of Agriculture, Beijing, 102206, China.ORCID https://orcid.org/0000-0003-3585-1430

Funding

2115 Talent Development Program of China Agricultural UniversityBiological Breeding-National Science and Technology Major Project 2023ZD0407503Biological Breeding-National Science and Technology Major Project 2023ZD0407504Hebei Province Science and Technology Plan 23227602ZNational Key R&D Program of China 2016YFA0100202National Key R&D Program of China 2022YFD1302201Natural Science Foundation of China 32370846Shijiazhuang City Science and Technology Project 221790262A
6 · The paper itself

Abstract

Capturing different pluripotent state stem cells from epiblast in vitro helps understand embryonic development and provides invaluable cell sources for basic research and regenerative medicine. Sheep are not only one of the most important livestock species in agriculture but also serve as an ideal preclinical model for studying human disease. Single-cell transcriptome analysis of sheep preimplantation embryos from embryonic day (E) 1 to E14 is performed to investigate the pluripotency changes of epiblast and elucidate the pluripotent regulation signaling. By combination of growth factors or inhibitors of JAK/STAT3, FGF, WNT, and TGF-β pathways in the culture medium, sheep formative and primed pluripotent stem cells (sfPSCs and spPSCs) are established respectively. The newly derived PSCs could maintain over 100 passages and differentiate into three germ layers. In addition, sfPSCs and spPSCs exhibit different molecular features, and sfPSCs have the ability of contribution to ICM and can be used as donor cells for producing cloned embryos efficiently. A cross-species comparison of early embryo development in mouse, pig and sheep illustrates the conservation of the epiblast naïve to primed state transition process and the divergence of the developmental events time points, the specific gene expression patterns and pluripotent regulation signaling. These studies are expected to improve our understanding of mammal early embryo development and present a reference for defining pluripotency.

Indexed as

BlastocystEmbryonic DevelopmentGerm LayersPluripotent Stem CellsAnimalsMiceSheepSwineembryo developmentepiblastpluripotencysheepsingle‐cell RNA seq

Identifiers

PMID40586282
PMCPMC12463017

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.