Evidence map›Paper›PMID 40585519›Full record

ArticleGhana medical journal2024

Analysis of Human Papillomavirus-HPV-18 and HPV-45 Type-specific variants in a contemporary cohort of individuals with cervical disease in Ghana.

Gladys Kaba, Andrew Stevenson, Samuel A Sakyi, Thomas O Konney, Nicholas A Titiloye, Samuel A Oppong, Kwabena Amo-Antwi, Francis Agyemang-Yeboah, Kate Cuschieri, Sheila V Graham

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Article in Ghana medical journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Gladys KabaDepartment of Molecular Medicine, School of Medical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana, West Africa.
Andrew StevensonCentre for Virus Research, School of Infection and Immunity, College of Medical, Veterinary and Life Sciences, Garscube Estate, University of Glasgow, Scotland, UK, G61 1QH.
Samuel A SakyiDepartment of Molecular Medicine, School of Medical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana, West Africa.
Thomas O KonneyDepartment of Obstetrics and Gynaecology, Komfo Anokye Teaching Hospital and Kwame Nkrumah University of Science and Technology, Kumasi, Ghana, West Africa.
Nicholas A TitiloyeDepartment of Pathology, School of Medical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana, West Africa.
Samuel A OppongDepartment of Obstetrics and Gynaecology, Korle-Bu Teaching Hospital and University of Ghana Medical School, Accra, Ghana, West Africa.
Kwabena Amo-AntwiDepartment of Obstetrics and Gynaecology, Komfo Anokye Teaching Hospital and Kwame Nkrumah University of Science and Technology, Kumasi, Ghana, West Africa.
Francis Agyemang-YeboahDepartment of Molecular Medicine, School of Medical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana, West Africa.
Kate CuschieriScottish HPV Reference Laboratory, Department of Laboratory Medicine, Royal Infirmary of Edinburgh, University of Edinburgh, Scotland, UK, EH16 4SA.
Sheila V GrahamCentre for Virus Research, School of Infection and Immunity, College of Medical, Veterinary and Life Sciences, Garscube Estate, University of Glasgow, Scotland, UK, G61 1QH.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Objectives: E6 and E7 DNA sequence Design: A cross-sectional study. Setting: Obstetrics/Gynaecology Directorate, Komfo Anokye Teaching Hospital, Kumasi and Department of Radiotherapy/Nuclear Medicine and the Family Planning Unit, Korle-Bu Teaching Hospital, Accra. Participants: 207 individuals referred with clinical suspicion of cervical cancer (CxCa) or confirmed CxCa/precancer cases. Methods: Cervical swabs were collected (from October 2018 to November 2020) from individuals, with L1 DNA positivity for HPV-18(40 samples) and/or HPV-45(28 samples), out of 207 samples tested for 24-HPV-genotypes. DNA was extracted from a convenience sample and HPV-E6/E7-PCR (33/40-HPV-18-+ve- or 20/28-HPV-45-+ve samples), sequencing, and BLAST analysis was carried out. Results: After PCR amplification, the E6/E7 gene regions of 26 out of 33(HPV-18+ve) samples and Conclusions: Our study confirms a dominance of HPV-45-sublineage-A1 and HPV-18-lineage-B (with rare occurrence of Africa-specific HPV-18-lineage-C) variants in Ghana. Our study provides preliminary data on E6/E7 SNPs of HPV-18 and HPV-45 lineages and sublineages among CxCa cases in Ghana. We hope our data will inform future studies on pattern and distribution of HPV type-specific nucleotide changes that can be useful for therapeutic intervention. Funding: UK Government, (GK) Commonwealth Split-Site Scholarship with research support grant, number GHCN201823. GK also received Ghana public universities senior members book & research allowance, for research activities carried out in Ghana.

Indexed as

Human papillomavirus 18Papillomavirus InfectionsUterine Cervical NeoplasmsAdultAgedCross-Sectional StudiesDNA, ViralFemaleGenotypeGhanaHuman Papillomavirus VirusesHumansMiddle AgedOncogene Proteins, ViralYoung AdultDNA, ViralOncogene Proteins, ViralHPV-18/HPV-45 variantsHuman papillomaviruslineage/sublineage

Identifiers

PMID40585519
PMCPMC12203745

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.