Evidence map›Paper›PMID 40585209›Full record

ArticleResearch square2025

Single-cell transcriptomics reveal differences between chorionic and basal plate cytotrophoblasts and trophoblast stem cells.

Robert Morey, Francesca Soncin, Sampada Kallol, Nirvay Sah, Zoe Manalo, Tony Bui, Jaroslav Slamecka, Virginia Chu Cheung, Don Pizzo, Daniela F Requena and 8 more

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PubMed Full text at PMC Publisher
In one paragraph

Article in Research square, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

18 authors.

Robert MoreyDepartment of Pathology, School of Medicine, University of California San Diego, La Jolla, 92093, CA, USA.ORCID https://orcid.org/0000-0001-8480-4564
Francesca SoncinDepartment of Pathology, School of Medicine, University of California San Diego, La Jolla, 92093, CA, USA.
Sampada KallolDepartment of Pathology, School of Medicine, University of California San Diego, La Jolla, 92093, CA, USA.
Nirvay SahDepartment of Pathology, School of Medicine, University of California San Diego, La Jolla, 92093, CA, USA.
Zoe ManaloDepartment of Pathology, School of Medicine, University of California San Diego, La Jolla, 92093, CA, USA.
Tony BuiDepartment of Pathology, School of Medicine, University of California San Diego, La Jolla, 92093, CA, USA.
Jaroslav SlameckaDepartment of Pathology, School of Medicine, University of California San Diego, La Jolla, 92093, CA, USA.ORCID https://orcid.org/0000-0003-3610-3223
Virginia Chu CheungDepartment of Pathology, School of Medicine, University of California San Diego, La Jolla, 92093, CA, USA.
Don PizzoDepartment of Pathology, School of Medicine, University of California San Diego, La Jolla, 92093, CA, USA.
Daniela F RequenaDepartment of Pathology, School of Medicine, University of California San Diego, La Jolla, 92093, CA, USA.
Ching-Wen ChangDepartment of Pathology, School of Medicine, University of California San Diego, La Jolla, 92093, CA, USA.
Omar FarahDepartment of Pathology, School of Medicine, University of California San Diego, La Jolla, 92093, CA, USA.
Ryan KittleDepartment of Pathology, School of Medicine, University of California San Diego, La Jolla, 92093, CA, USA.ORCID https://orcid.org/0009-0007-1147-5190
Kelly LamDepartment of Pathology, School of Medicine, University of California San Diego, La Jolla, 92093, CA, USA.
Morgan MeadsDepartment of Pathology, School of Medicine, University of California San Diego, La Jolla, 92093, CA, USA.
Mariko HoriiDepartment of Pathology, School of Medicine, University of California San Diego, La Jolla, 92093, CA, USA.ORCID https://orcid.org/0000-0002-4996-6792
Kathleen M FischCenter for Perinatal Discovery, University of California San Diego, La Jolla, 92093, CA, USA.ORCID https://orcid.org/0000-0002-0117-7444
Mana M ParastDepartment of Pathology, School of Medicine, University of California San Diego, La Jolla, 92093, CA, USA.ORCID https://orcid.org/0000-0001-5963-2246

Funding

UC San Diego Clinical and Translational Research InstituteUL1TR001442 · NCATS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI FIRESTEIN, GARY S, HOGARTH, MICHAEL · 2015 to 2024
$88.3M
TRAINING IN REPRODUCTIVE SCIENCEST32HD007203 · NICHD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI PAMELA L MELLON · 1988 to 2026
$4.8M
Cellular Atlas of the Human Placenta: Structure-Function Relationships and their Implications for Placental DysfunctionR01HD104805 · NICHD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI PARAST, MANA M · 2021 to 2025
$2.8M
Trophoblast progenitor heterogeneity and function in normal and Trisomy 21-affected placentaeR01HD111787 · NICHD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Mana M Parast · 2023 to 2026
$2.6M
Role of VGLL1 in human placental development and trophoblast specificationR01HD096260 · NICHD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI SONCIN, FRANCESCA · 2019 to 2023
$2.5M
Human Trophoblast Stem Cells: the In Vivo Niche and Relationship to Pluripotent Stem CellsR01HD089537 · NICHD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI PARAST, MANA M · 2017 to 2021
$2.4M
Modeling Preeclampsia Using Human Pluripotent Stem CellsR00HD091452 · NICHD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI HORII, MARIKO · 2020 to 2022
$737k
Illumina NovaSeq 6000 Sequencing SystemS10OD026929 · OD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI JEPSEN, KRISTEN LYNN · 2019 to 2019
$600k
NCATS NIH HHS UL1 TR001442NICHD NIH HHS R00 HD091452NICHD NIH HHS R01 HD089537NICHD NIH HHS R01 HD096260NICHD NIH HHS R01 HD104805NICHD NIH HHS R01 HD111787NICHD NIH HHS T32 HD007203NIH HHS S10 OD026929
6 · The paper itself

Abstract

Cytotrophoblast (CTB) of the early gestation human placenta are bipotent progenitor epithelial cells, which can differentiate into invasive extravillous trophoblast (EVT) and multinucleated syncytiotrophoblast (STB). Trophoblast stem cells (TSC), derived from early first trimester placentae, have also been shown to be bipotential; however, their cell-of-origin has not been identified. In this study, we set out to probe the transcriptional diversity of early and late first trimester villous CTB (vCTB) and compare these to TSC. To this end, we performed single-cell RNA sequencing (scRNA-seq) on placental villous tissue from early (6-8 weeks) and late (12-14 weeks) first trimester placentae; we also evaluated CTB within basal (maternal) and chorionic (fetal) regions of early first trimester placenta, both by scRNA-seq and GeoMx-based spatial transcriptomics. Finally, we performed scRNA-seq on three TSC lines, derived from 6-8 week gestation placentae, as well as on early first trimester CTB at several timepoints during TSC derivation. We found notable distinctions within CTB clusters based on gestational age, further influenced by location near the basal or chorionic plates. We identified trophoblast states representing "initial state" vCTB (

Indexed as

CytotrophoblastPlacentaTrophoblastTrophoblast stem cells

Identifiers

PMID40585209
PMCPMC12204482

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.