Effect of Acute Administration of Caffeine on Neuropathic Pain and the Role of Nitric Oxide Pathway in an Animal Model of Chronic Constriction Injury.

Introduction: Partial peripheral nerve injury often results in chronic pain, including hyperalgesia and allodynia. Caffeine, as a non-selective antagonist of adenosine receptors (ARs), has protective effects on neuropathic pain. Since nitric oxide (NO) is partially involved in the central effects of caffeine, we investigated the effects of acute caffeine administration on neuropathic pain, focusing on A Methods: Following chronic constriction injury (CCI), male Wistar rats were administered caffeine (10, 50, and 100 mg/kg). Also, groups of animals received L-NAME (30 mg/kg) or L-arginine (100 mg/kg) either alone or before treatment with 50 mg/kg of caffeine. Rats were tested for hyperalgesia and allodynia at 4, 7, 14, 21, and 28 days following CCI. Results: Administration of 10 mg/kg of caffeine significantly increased cold allodynia, while 50 and 100 mg/kg of caffeine decreased mechanical allodynia and thermal hyperalgesia. Pre-treatment with L-NAME before caffeine administration decreased cold and mechanical allodynia and thermal hyperalgesia. Treatment with L-arginine before caffeine administration increased thermal hyperalgesia and decreased cold allodynia. Conclusion: The present data show that caffeine dose-dependently affects the pro-analgesic or anti-analgesic states in the CCI model.