Evidence map›Paper›PMID 40583867›Full record

ArticleCytotechnology2025

Expression and localization of lysosomal-associated membrane protein 1- and perforin-based hybrid molecules in the murine cytotoxic T cell line CTLL-2.

Yuto Ueno, Rikako Yamaguchi, Yumiko Tanaka, Yasunobu Miyake, Keiko Takaki, Ayaka Nakao, Ryota Ozaki, Yuka Yokota, Itsuki Fukai, Sara Kuwada and 5 more

Abstract read
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In one paragraph

Article in Cytotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Yuto Ueno *Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585 Japan.
Rikako Yamaguchi *Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585 Japan.
Yumiko Tanaka *Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585 Japan.
Yasunobu MiyakeDivision of Molecular and Cellular Immunoscience, Department of Biomolecular Sciences, Faculty of Medicine, Saga University, Saga, 849-8501 Japan.
Keiko TakakiDepartment of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585 Japan.
Ayaka NakaoDepartment of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585 Japan.
Ryota OzakiDepartment of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585 Japan.
Yuka YokotaDepartment of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585 Japan.
Itsuki FukaiDepartment of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585 Japan.
Sara KuwadaDepartment of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585 Japan.
Natsuki FukuokaDepartment of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585 Japan.
Naoaki OkudaDepartment of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585 Japan.
Ai NishidaDepartment of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585 Japan.
Tomoo ShibaDepartment of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585 Japan.
Takao KataokaDepartment of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585 Japan.ORCID 0000-0002-6000-4500

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cytotoxic T lymphocytes (CTL) and natural killer cells harbor lytic granules as secretory lysosomes. Lytic granules contain perforin as a soluble protein that forms pores and is essential for target cell lysis. Lysosomal-associated membrane protein 1 (LAMP1) is a single transmembrane protein that is enriched in lysosomes and is also present in lytic granules. In this study, to investigate the feasibility of membrane-integral markers for lytic granules, LAMP1- and perforin-based hybrid molecules were constructed and their expression and localization were examined in the murine cytotoxic T-cell line CTLL-2. Stable CTLL-2 transfectants were established by nucleofection using linearized vectors encoding human LAMP1 (1-417) fused to enhanced green fluorescent protein (EGFP) and FLAG (LAMP1-EGFP-FLAG) and human perforin (1-555) fused to human LAMP1 (325-417), EGFP, and FLAG (Perforin-LAMP1-EGFP-FLAG). Confocal microscopy showed that LysoTracker Red colocalized with LAMP1-EGFP-FLAG and Perforin-LAMP1-EGFP-FLAG, and Perforin-LAMP1-EGFP-FLAG showed a broader cytoplasmic distribution than LAMP1-EGFP-FLAG. A Percoll density gradient centrifugation analysis revealed that LAMP1-EGFP-FLAG and Perforin-LAMP1-EGFP-FLAG were distributed in fractions associated with the endogenous expression of perforin and LAMP1. The effects of glycosylation inhibitors on the expression and intracellular transport of LAMP1-EGFP-FLAG were also investigated. Concanamycin A, an inhibitor of vacuolar-type H

Indexed as

Concanamycin ACTLL-2LAMP1Lytic granuleNucleofectorPerforin

Identifiers

PMID40583867
PMCPMC12198104

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.