Evidence map›Paper›PMID 40583153›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025

Single-Droplet Dual-Target Quantification of circRNA Biomarkers for Colorectal Cancer Screening.

Jingsong Xu, Yu Liu, Junheng Zhang, Shuang Yang, Tianming Li, Haiqian Huang, Qian Liu, Hengliang Wang, Li Cao, Zhenghua An and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
  4. Article
  5. Review
  6. Article
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Jingsong XuDepartment of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.
Yu LiuDepartment of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.
Junheng ZhangDepartment of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.
Shuang YangDepartment of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.
Tianming LiDepartment of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.
Haiqian HuangDepartment of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.
Qian LiuDepartment of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.
Hengliang WangState Key Laboratory of Surface Physics, Department of Physics, Fudan University, Shanghai, 200438, China.
Li CaoDepartment of Pathology, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai, 200137, China.
Zhenghua AnState Key Laboratory of Surface Physics, Department of Physics, Fudan University, Shanghai, 200438, China.
Min LiDepartment of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.
Hua WangDepartment of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.ORCID https://orcid.org/0000-0002-7319-6249

Funding

Clinical Excellence project of Shanghai Key Laboratory of Nucleic Acid Chemistry and Nanomedicine 2024ZY008Cross-Research Fund of Biomedical Engineering of Shanghai Jiaotong University YG2024LC02National Key Research and Development Program 2023YFC2306200National Natural Science Foundation of China 82472374
6 · The paper itself

Abstract

Circular RNAs (circRNAs) play a crucial role in the oncogenesis, progression, and chemoresistance of tumors. Here, two plasma circRNA biomarkers, circCK1γ3 and circWDR37 is identified and validated. Simultaneous analysis of these biomarkers has the potential to significantly improve the sensitivity of colorectal cancer (CRC) screening. Leveraging the distinct base-cutting preferences of these two Cas proteins, it is successfully expressed and purified LwaCas13a and PsmCas13b, and developed a one-pot Cas13a/13b-RPA assay for the simultaneous detection of circCK1γ3 and circWDR37. To achieve precise detection of circCK1γ3 and circWDR37, the Casµchip, which integrates droplet generation and digital quantification functionalities is designed and fabricated. The Casµchip enables the simultaneous detection of circCK1γ3 and circWDR37 within 70 min. This method exhibits excellent repeatability and high specificity, with a detection limit of 100 copies/reaction. The correlation coefficients for circWDR37 and circCK1γ3 detected by Casµchip and qPCR are 0.9113 and 0.8933, respectively. Casµchip analysis is conducted on a cohort of 60 clinical samples, and the findings indicate that this methodology holds promise for the early detection of colorectal cancer. Casµchip represents the profound integration of CRISPR diagnostics and microfluidic technology, offering a novel paradigm characterized by rapid, precise, and user-friendly features for molecular diagnosis.

Indexed as

Biomarkers, TumorColorectal NeoplasmsEarly Detection of CancerRNA, CircularHumansBiomarkers, TumorRNA, Circularcancer screeningcircRNAdual‐CRISPRmicrofluidic chip

Identifiers

PMID40583153
PMCPMC12520571

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.