Evidence map›Paper›PMID 40581888›Full record

ArticleInflammopharmacology2025

5-lipoxygenase is critically involved in joint inflammation and damage in experimental osteoarthritis.

Adryan Aparecido Silva, Laura Lima Oliveira, Celso Martins Queiroz-Junior, Beatriz Ferreira Carvalho Versiani Caldeira, Anna Clara Bento Reis Soares, Gabrielly Carvalho Mattos, Amanda Dias Braga, Paula Lima Bosi, Amanda Maria Sena Reis, Mauro Martins Teixeira and 1 more

Abstract read
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In one paragraph

Article in Inflammopharmacology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Adryan Aparecido Silva *Department of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
Laura Lima Oliveira *Department of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
Celso Martins Queiroz-JuniorDepartment of Morphology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
Beatriz Ferreira Carvalho Versiani CaldeiraDepartment of Pathology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
Anna Clara Bento Reis SoaresDepartment of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
Gabrielly Carvalho MattosDepartment of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
Amanda Dias BragaDepartment of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
Paula Lima BosiDepartment of Morphology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
Amanda Maria Sena ReisDepartment of Pathology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
Mauro Martins TeixeiraDepartment of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
Flávio Almeida AmaralDepartment of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil. famaral@icb.ufmg.br.ORCID http://orcid.org/0000-0002-1695-0612

Funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico 403767/2021-0
6 · The paper itself

Abstract

objectivesTo investigate the role of 5-lipoxygenase (5-Lox) in driving joint inflammation and damage in a model of osteoarthritis induced by collagenase in mice.

methodsAn osteoarthritis model was established in adult SV129 wild-type (WT) and 5-Lox-deficient (5-Lox

resultsThe injection of collagenase increased the protein expression of 5-Lox at 7-21 days after this challenge, returning to the basal level on day 42. The expression of Lta4-hydrolase, a critical enzyme involved in leukotriene B4 production, also increased with time after collagenase injection. Compared with WT mice, 5-Lox

conclusion5-Lox is critical for joint inflammation and degradation in a mouse model of osteoarthritis. Inhibiting 5-Lox with Zileuton effectively prevented joint damage and inflammation, even when it was given weeks after the inciting stimulus, suggesting that it is a potential strategy to limit the progression of osteoarthritis.

Indexed as

Arachidonate 5-LipoxygenaseInflammationOsteoarthritisAnimalsCollagenasesDisease Models, AnimalHydroxyureaJointsLipoxygenase InhibitorsMaleMiceMice, KnockoutArachidonate 5-LipoxygenaseCollagenasesHydroxyureaLipoxygenase Inhibitorszileuton5-LipoxygenaseCartilageInflammationJoint damageOsteoarthritisZileuton

Identifiers

PMID40581888

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.