ArticlePlant communications2025
Broad application of the ALFA tagging technology for in planta nanobody-based imaging and biochemical characterization of plant proteins.
Article in Plant communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Canonical tyrosine-based motifs are required for constitutive endocytosis and polarity of IRT1 and contribute to metal uptake.The Plant journal : for cell and molecular biology · 2025Article
- Metal-sensing properties of the disordered loop from the Arabidopsis metal transceptor IRT1.The Biochemical journal · 2025Article
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Authors and funding
6 authors.
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Abstract
Epitope tags are widely used for detecting, modifying, or purifying proteins of interest, but their range of application is often limited. Recently, the rationally designed ALFA tag and its ALFA nanobody have expanded the repertoire of epitope tags and emerged as a highly versatile system characterized in various animal models, outperforming existing tags. Here, we evaluated the ALFA tag/ALFA nanobody technology in plants and demonstrated its application for in planta protein detection across multiple compartments and cellular structures, protein-protein interaction studies, protein immunoprecipitation, induced-proximity approaches, and super-resolution microscopy. Most importantly, we highlight the potential of the ALFA tagging technology for proteins that are difficult to tag due to topological or functional constraints. We provide proof of concept for the ALFA tag technology in the detection and functional analysis of the Arabidopsis IRT1 Fe transporter. Overall, this versatile and validated toolbox of ALFA tag and ALFA nanobody applications will serve as a valuable resource for functional studies in plants.
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