Evidence map›Paper›PMID 40581780›Full record

ArticleBioinformatics (Oxford, England)2025

NGPINT V3: a containerized orchestration Python software for discovery of next-generation protein-protein interactions.

Schuyler D Smith, Valeria Velásquez-Zapata, Roger P Wise

Abstract read
In one paragraph

Article in Bioinformatics (Oxford, England), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Schuyler D SmithDepartment of Plant Pathology, Entomology, and Microbiology, Iowa State University, Ames, IA 50011, United States.ORCID 0000-0001-5720-5611
Valeria Velásquez-ZapataDepartment of Plant Pathology, Entomology, and Microbiology, Iowa State University, Ames, IA 50011, United States.ORCID 0000-0002-2756-2156
Roger P WiseDepartment of Plant Pathology, Entomology, and Microbiology, Iowa State University, Ames, IA 50011, United States.ORCID 0000-0001-7786-1528

Funding

National Institute of Food and Agriculture 2020-67013-31184
6 · The paper itself

Abstract

summaryBatch yeast two-hybrid (Y2H) assays, leveraged with next-generation sequencing, have afforded successful innovations for the analysis of protein-protein interactions. NGPINT is a Conda-based software designed to process the millions of raw sequencing reads resulting from Y2H-next-generation interaction screens. Over time, increasing compatibility and dependency issues have prevented clean NGPINT installation and operation. A system-wide update was essential to continue effective use with its companion software, Y2H-SCORES. We present NGPINT V3, a containerized implementation built with both Singularity and Docker, allowing accessibility across virtually any operating system and computing environment. AVAILABILITY AND IMPLEMENTATION: This update includes streamlined dependencies and container images hosted on Sylabs (https://cloud.sylabs.io/library/schuyler/ngpint/ngpint) and Dockerhub (https://hub.docker.com/r/schuylerds/ngpint), facilitating easier adoption and integration into high-throughput and cloud-computing workflows. Full instructions and software can be also found in the GitHub repository https://github.com/Wiselab2/NGPINT_V3 and Zenodo https://doi.org/10.5281/zenodo.15256036.

Indexed as

High-Throughput Nucleotide SequencingProtein Interaction MappingSoftwareComputational BiologyProgramming LanguagesTwo-Hybrid System Techniques

Identifiers

PMID40581780
PMCPMC12208055

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.