Fast and Accurate Enrichment of Canid Excrement for Species* and Other Analyses (FAECES*) Method for Noninvasive Genotyping of Field-Collected Canid Scat.

Lillian D Parker, Jesús E Maldonado, Michael G Campana

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Lillian D ParkerLaboratories of Molecular Anthropology and Microbiome Research, Department of Anthropology, University of Oklahoma, Norman, OK, USA.

Jesús E MaldonadoCenter for Conservation Genomics, Smithsonian National Zoo and Conservation Biology Institute, Washington, DC, USA.

Michael G CampanaCenter for Conservation Genomics, Smithsonian National Zoo and Conservation Biology Institute, Washington, DC, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The FAECES protocol details a method for obtaining multi-locus SNP genotypes from noninvasively collected scat samples. It employs a capture enrichment technique that can be adapted for various vertebrate species. The workflow encompasses probe design using BaitsTools, DNA extraction using the Qiagen DNeasy PowerSoil Pro kit, genomic DNA library preparation via the Santa Cruz Reaction (SCR) protocol, in-solution hybridization capture with myBaits probes, and comprehensive bioinformatic analysis utilizing tools such as FastQC, TrimGalore, Nextflow, and SNPRelate. The protocol aims to differentiate between species, identify individuals, and determine sex from scat samples, emphasizing flexibility and adaptability for diverse research applications.

Indexed as

FecesGenotyping TechniquesAnimalsComputational BiologyDNAGenotypePolymorphism, Single NucleotideDNABioinformaticsCapture enrichmentGenotyping identificationHybridizationNoninvasive samplingSex determinationSNP genotyping

Identifiers

PMID40580297

DOI10.1101/gr.107524.110

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.