Evidence map›Paper›PMID 40580295›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Long-Range Targeted Nanopore Ligation Sequencing Workflow for Targeted Amplicon Sequencing of Secondary Metabolite Gene.

Anthony Torres, Reginald Gaudino

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Anthony TorresCannabis Research Institute, Discovery Partners Institute, University of Illinois System, Chicago, IL, USA.ORCID https://orcid.org/0000-0003-3631-3079
Reginald GaudinoFront Range Biosciences, Lafayette, CO, USA.ORCID https://orcid.org/0000-0001-5902-5606

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This chapter presents protocols for advanced genotyping and genetic analysis in plant science, utilizing DNA extracted from FTA samples and leaf tissue. Targeted long range PCR is employed to uncover allelic and structural variations within genes of interest, while targeted long-read nanopore sequencing offers a comprehensive view of gene targets in their native and enriched forms. The protocol for nanopore sequencing encompasses DNA preparation, barcoding of up to 96 samples, adapter ligation, purification, and sequencing, culminating in the generation of raw data files (pod5 or fastq) suitable for further analysis. Together, these methods provide a powerful toolkit for plant diagnostics and breeding.

Indexed as

High-Throughput Nucleotide SequencingNanopore SequencingPlantsSequence Analysis, DNADNA, PlantNanoporesPolymerase Chain ReactionWorkflowDNA, PlantAgricultural biotechnologyDNA barcodingFTA samplesGenotypingLeaf tissueLong-read nanopore sequencingMinION sequencer long-read DNAPlant diagnosticsTargeted long-range PCR

Identifiers

PMID40580295

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.