Evidence map›Paper›PMID 40580284›Full record

ReviewMethods in molecular biology (Clifton, N.J.)2025

Isothermal Amplification Techniques: An Emerging Tool for On-Site Detection of Phytopathogens in Field Conditions.

C Manjunatha, K Aditya, M K Prasannakumar, D Pramesh, P Buella, Swathi S Patil, Gopal Venkateshbabu, J Harish, R Karan, H V Ramegowda

Abstract readReview
PubMed Publisher
In one paragraph

Review in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Buruli ulcer.Nature reviews. Disease primers · 2025
    Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

C ManjunathaDivision of Genomic Resources, ICAR-National Bureau of Agricultural Insect Resources, Bengaluru, India.
K AdityaDivision of Genomic Resources, ICAR-National Bureau of Agricultural Insect Resources, Bengaluru, India.
M K PrasannakumarPathoGenOmics Lab, Department of Plant Pathology, University of Agricultural Sciences, Bengaluru, India.
D PrameshAgricultural Research Station, Gangavathi, University of Agricultural Sciences, Raichur, India.
P BuellaPathoGenOmics Lab, Department of Plant Pathology, University of Agricultural Sciences, Bengaluru, India.
Swathi S PatilPathoGenOmics Lab, Department of Plant Pathology, University of Agricultural Sciences, Bengaluru, India.
Gopal VenkateshbabuPathoGenOmics Lab, Department of Plant Pathology, University of Agricultural Sciences, Bengaluru, India.
J HarishPathoGenOmics Lab, Department of Plant Pathology, University of Agricultural Sciences, Bengaluru, India.
R KaranPathoGenOmics Lab, Department of Plant Pathology, University of Agricultural Sciences, Bengaluru, India.
H V RamegowdaAgricultural Research Station, Gangavathi, University of Agricultural Sciences, Raichur, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This chapter reviews various isothermal amplification techniques, which are alternative to PCR for detecting plant pathogens. These methods, including NASBA, SDA, LAMP, HDA, and RPA, amplify nucleic acids at a constant temperature, making them potentially more suitable for point-of-care (POC) applications. The review compares these techniques regarding their mechanisms, advantages, and limitations, highlighting their potential for rapid, cost-effective, and on-site plant disease diagnosis, including the integration of these techniques with CRISPR-Cas systems and lab-on-a-chip technologies.

Indexed as

Molecular Diagnostic TechniquesNucleic Acid Amplification TechniquesPlant DiseasesPlantsCRISPR-Cas SystemsPoint-of-Care SystemsCRISPR-CasHDA (Helicase-dependent amplification)Isothermal amplificationLAMP (Loop-mediated isothermal amplification)Molecular diagnosticsNASBA (Nucleic acid sequence-based amplification)Nucleic acid amplificationPCR alternativePlant disease detectionPlant pathogensPoint-of-care (POC) diagnosticsRPA (Recombinase polymerase amplification)SDA (Strand displacement amplification)

Identifiers

PMID40580284

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.