Evidence map›Paper›PMID 40580270›Full record

ArticleApplied biochemistry and biotechnology2025

Transcriptomic Analysis of Lipid Accumulation in Mucor circinelloides WJ11 Under Submerged and Solid-State Fermentation.

Bushra Iqbal, Muhammad Tariq Saeed, Tahira Naz, Hassan Mohamed, Wenrui Dang, Asmaa S Ramadan, Adel Eltoukhy, Huaiyuan Zhang, Yuanda Song

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Article in Applied biochemistry and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Bushra IqbalColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, 255000, China.
Muhammad Tariq SaeedColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, 255000, China.
Tahira NazColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, 255000, China.
Hassan MohamedColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, 255000, China.
Wenrui DangColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, 255000, China.
Asmaa S RamadanColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, 255000, China.
Adel EltoukhyColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, 255000, China.
Huaiyuan ZhangColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, 255000, China. zhyuan004@126.com.
Yuanda SongColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, 255000, China. ysong@sdut.edu.cn.ORCID http://orcid.org/0000-0002-8565-6724

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The oleaginous filamentous fungus Mucor circinelloides was extensively investigated for bio-oil production using submerged fermentation (SmF) and solid-state fermentation (SSF) techniques. SSF fermentation may offer advantages over SmF, largely due to the fungus's unique filamentous structure and the lower costs associated with this strategy. In this study, M. circinelloides strain WJ11 exhibited lower fatty acid (FA) content, reaching up to 12% in SSF compared with 24.1% in SmF after 120 h. Furthermore, transcriptomic analysis observed that 7615 genes were differentially expressed between the two fermentation methods, with 3891 genes upregulated and 3724 downregulated in SSF. Notably, genes encoding triglyceride-related synthases were significantly downregulated in WJ11. The mRNA levels of ATP-citrate lyase were also reduced, indicating a decreased supply of acetyl-CoA for fatty acid biosynthesis in SSF. Additionally, isoleucine and leucine metabolism do not contribute to acetyl-CoA production, further hindering FA synthesis. For NADPH production, which is crucial for FA biosynthesis, the pentose phosphate pathway (PPP) was significantly downregulated, and the activity of NADP

Indexed as

FermentationGene Expression ProfilingLipid MetabolismMucorTranscriptomeATP Citrate (pro-S)-LyaseFatty AcidsATP Citrate (pro-S)-LyaseFatty AcidsLipid biosynthesisMucor circinelloidesSmFSSFTranscriptomics

Identifiers

PMID40580270

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.