Evidence map›Paper›PMID 40579675›Full record

ArticleIn vitro cellular & developmental biology. Animal2026

Efficient cryopreservation of Antillean manatee skin-derived somatic cells via reduced intracellular cryoprotectant concentration.

Luanna Lorenna Vieira Rodrigues, Lhara Ricarliany Medeiros de Oliveira, Yasmin Beatriz França Moura, Yara Letícia Frutuoso E Silva, João Vitor da Silva Viana, Leonardo Vitorino Costa de Aquino, Radan Elvis Matias de Oliveira, Fernanda Loffler Niemeyer Attademo, Fábia Oliveira Luna, Alexsandra Fernandes Pereira

Abstract read
PubMed Publisher
In one paragraph

Article in In vitro cellular & developmental biology. Animal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Biobanking biodiversity: recent strategies, challenges, and opportunities.The Journal of reproduction and development · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Luanna Lorenna Vieira RodriguesLaboratory of Animal Biotechnology, Federal Rural University of Semi-Arid, Av. Francisco Mota, Mossoró, RN, 57259625-900, Brazil.
Lhara Ricarliany Medeiros de OliveiraLaboratory of Animal Biotechnology, Federal Rural University of Semi-Arid, Av. Francisco Mota, Mossoró, RN, 57259625-900, Brazil.
Yasmin Beatriz França MouraLaboratory of Animal Biotechnology, Federal Rural University of Semi-Arid, Av. Francisco Mota, Mossoró, RN, 57259625-900, Brazil.
Yara Letícia Frutuoso E SilvaLaboratory of Animal Biotechnology, Federal Rural University of Semi-Arid, Av. Francisco Mota, Mossoró, RN, 57259625-900, Brazil.
João Vitor da Silva VianaLaboratory of Animal Biotechnology, Federal Rural University of Semi-Arid, Av. Francisco Mota, Mossoró, RN, 57259625-900, Brazil.
Leonardo Vitorino Costa de AquinoLaboratory of Animal Biotechnology, Federal Rural University of Semi-Arid, Av. Francisco Mota, Mossoró, RN, 57259625-900, Brazil.
Radan Elvis Matias de OliveiraLaboratory of Applied Animal Morphophysiology, Federal Rural University of Semi-Arid, Mossoró, Brazil.
Fernanda Loffler Niemeyer AttademoLaboratory of Animal Biotechnology, Federal Rural University of Semi-Arid, Av. Francisco Mota, Mossoró, RN, 57259625-900, Brazil.
Fábia Oliveira LunaNational Center for Research and Conservation of Aquatic Mammals By Chico Mendes Institute for Biodiversity Conservation, São Paulo, Brazil.
Alexsandra Fernandes PereiraLaboratory of Animal Biotechnology, Federal Rural University of Semi-Arid, Av. Francisco Mota, Mossoró, RN, 57259625-900, Brazil. alexsandra.pereira@ufersa.edu.br.ORCID http://orcid.org/0000-0003-2137-854X

Funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico 309078/2021-0Coordenação de Aperfeiçoamento de Pessoal de Nível Superior 001
6 · The paper itself

Abstract

The declining population of the Antillean manatees has prompted interest in developing conservation strategies, including somatic cell cryopreservation. However, the type and concentration of intracellular cryoprotectant agents (CPAs) are limiting factors for its success. Therefore, we evaluated three concentrations (5, 8, 10%) of dimethyl sulfoxide (Me

Indexed as

CryopreservationCryoprotective AgentsSkinAnimalsApoptosisCell ProliferationCell SurvivalDimethyl SulfoxideEthylene GlycolMembrane Potential, MitochondrialReactive Oxygen SpeciesCryoprotective AgentsDimethyl SulfoxideEthylene GlycolReactive Oxygen SpeciesConservation strategiesCryobankingDermal cellsSireniansSlow freezing

Identifiers

PMID40579675

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.