Evidence map›Paper›PMID 40579490›Full record

ArticleNature biomedical engineering2025

Scalable production of human cortical organoids using a biocompatible polymer.

Genta Narazaki, Yuki Miura, Sergey D Pavlov, Mayuri Vijay Thete, Julien G Roth, Merve Avar, Sungchul Shin, Ji-Il Kim, Zuzana Hudacova, Sarah C Heilshorn and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature biomedical engineering, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Genta Narazaki *Department of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0003-3063-2570
Yuki Miura *Department of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0002-1410-3612
Sergey D PavlovDepartment of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0002-0496-2042
Mayuri Vijay TheteDepartment of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA, USA.
Julien G RothStanford Brain Organogenesis, Wu Tsai Neuroscience Institute, Stanford, CA, USA.ORCID http://orcid.org/0000-0002-7560-3258
Merve AvarDepartment of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0003-4665-5558
Sungchul ShinStanford Brain Organogenesis, Wu Tsai Neuroscience Institute, Stanford, CA, USA.
Ji-Il KimDepartment of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0002-5710-7090
Zuzana HudacovaDepartment of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA, USA.
Sarah C HeilshornStanford Brain Organogenesis, Wu Tsai Neuroscience Institute, Stanford, CA, USA.ORCID http://orcid.org/0000-0002-9801-6304
Sergiu P PașcaDepartment of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA, USA. spasca@stanford.edu.ORCID http://orcid.org/0000-0002-3216-3248

Funding

Gaining insight into psychiatric disease by engineering piece by piece the human brain in vitro.R01MH107800 · NIMH · STANFORD UNIVERSITY · PI PASCA, SERGIU · 2015 to 2024
$5.6M
Gaining insight into psychiatric disease by engineering piece by piece the human brain in vitroR37MH107800 · NIMH · STANFORD UNIVERSITY · PI PASCA, SERGIU · 2025 to 2025
$3.8M
An Engineered Bioprinting Platform to Study Neural Migration in AssembloidsR01MH137333 · NIMH · STANFORD UNIVERSITY · PI Sarah C Heilshorn · 2025 to 2026
$1.4M
Engineered biomaterials to modulate cell-cell signaling for the robust expansion of stem cellsR01EB027171 · NIBIB · STANFORD UNIVERSITY · PI HEILSHORN, SARAH C · 2019 to 2022
$1.4M
NIBIB NIH HHS R01 EB027171NIMH NIH HHS R01 MH107800NIMH NIH HHS R01 MH137333NIMH NIH HHS R37 MH107800
6 · The paper itself

Abstract

The generation of neural organoids from human pluripotent stem cells holds great promise in modelling disease and screening drugs, but current approaches are difficult to scale due to undesired organoid fusion. Here we develop a scalable cerebral cortical organoid platform by screening biocompatible polymers that prevent the fusion of organoids cultured in suspension. We identify a cost-effective polysaccharide that increases the viscosity of the culture medium, significantly enhancing the yield of cortical organoids while preserving key features such as regional patterning, neuronal morphology and functional activity. We further demonstrate that this platform enables straightforward screening of 298 FDA-approved drugs and teratogens for growth defects using over 2,400 cortical organoids, uncovering agents that disrupt organoid growth and development. We anticipate this approach to provide a robust and scalable system for modelling human cortical development, and facilitate efficient compound screening for neuropsychiatric disorders-associated phenotypes.

Indexed as

Biocompatible MaterialsCerebral CortexOrganoidsPolymersCell Culture TechniquesCell DifferentiationCulture MediaHumansInduced Pluripotent Stem CellsNeuronsPluripotent Stem CellsBiocompatible MaterialsCulture MediaPolymers

Identifiers

PMID40579490
PMCPMC12811936

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.