Evidence map›Paper›PMID 40579472›Full record

ArticleNature nanotechnology2025

Nanopore detection of single-nucleotide RNA mutations and modifications with programmable nanolatches.

Yunxuan Li, Siong Chen Meng, Yesheng Wang, Casey M Platnich, Max K Earle, Elli Mylona, Plamena Naydenova, Stephen Baker, Jinbo Zhu, Ulrich F Keyser

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature nanotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. DNA Nanoarray for Multiplexed RNA Detection With Single-Molecule Readout.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
  3. Review
  4. Article
  5. Supramolecular Interactions Modulate RNA:DNA Folding Observed via Nanopore Sensing.Angewandte Chemie (International ed. in English) · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yunxuan LiCavendish Laboratory, University of Cambridge, Cambridge, UK.ORCID http://orcid.org/0009-0005-8362-5173
Siong Chen MengCavendish Laboratory, University of Cambridge, Cambridge, UK.ORCID http://orcid.org/0009-0006-9975-3951
Yesheng WangSchool of Biomedical Engineering, Faculty of Medicine, Dalian University of Technology, Dalian, China.
Casey M PlatnichCavendish Laboratory, University of Cambridge, Cambridge, UK.ORCID http://orcid.org/0000-0002-3634-4580
Max K EarleCavendish Laboratory, University of Cambridge, Cambridge, UK.
Elli MylonaCambridge Institute of Therapeutic Immunology and Infectious Disease, Jeffery Cheah Biomedical Centre, Cambridge Biomedical Campus, University of Cambridge, Cambridge, UK.ORCID http://orcid.org/0000-0002-2638-0713
Plamena NaydenovaCambridge Institute of Therapeutic Immunology and Infectious Disease, Jeffery Cheah Biomedical Centre, Cambridge Biomedical Campus, University of Cambridge, Cambridge, UK.
Stephen BakerA*STAR Infectious Diseases Labs, Agency for Science, Technology and Research, Singapore, Singapore.
Jinbo ZhuCavendish Laboratory, University of Cambridge, Cambridge, UK. jinbozhu@dlut.edu.cn.ORCID http://orcid.org/0000-0003-4935-825X
Ulrich F KeyserCavendish Laboratory, University of Cambridge, Cambridge, UK. ufk20@cam.ac.uk.ORCID http://orcid.org/0000-0003-3188-5414

Funding

Dalian University of Technology (DUT) 2023JJ12SN032EC | Horizon 2020 Framework Programme (EU Framework Programme for Research and Innovation H2020) 964995National Natural Science Foundation of China (National Science Foundation of China) 62471081Wellcome TrustWellcome Trust (Wellcome) 215515/Z/19/Z
6 · The paper itself

Abstract

RNA mutations and modifications have been implicated in a wide range of pathophysiologies. However, current RNA detection methods are hindered by data complexity and error-prone protocols, restricting their widespread use. Here we present a solid-state nanopore-based approach, RNA single-nucleotide characterization and analysis nanolatch (RNA-SCAN) system, which simplifies the detection of nucleotide mutations and modifications in RNA with high resolution. Using phage RNA as a template, we tested multiple sequences and chemical modifications on nanolatches, allowing the detection of mismatches caused by nucleotide mutations through significant changes in positive event ratios using single-molecule nanopore measurements. This approach is also sensitive to modifications that either strengthen or weaken the interaction between the target RNA sequence and the nanolatch. As a proof-of-concept, we demonstrate successful discrimination of Escherichia coli and Salmonella spp. from total RNA based on nucleotide variations in their 16S rRNA, as well as quantification of different Salmonella spp. and detection of m

Indexed as

MutationNanoporesNanotechnologyRNARNA, BacterialEscherichia coliPolymorphism, Single NucleotideRNA, Ribosomal, 16SRNA, ViralSalmonellaRNARNA, BacterialRNA, Ribosomal, 16SRNA, Viral

Identifiers

PMID40579472
PMCPMC12534182

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.