Evidence map›Paper›PMID 40578838›Full record

ArticleProtein & cell2025

Optimized derivation and culture system of human naïve pluripotent stem cells with enhanced DNA methylation status and genomic stability.

Yan Bi, Jindian Hu, Tao Wu, Zhaohui Ouyang, Tan Lin, Jiaxing Sun, Xinbao Zhang, Xiaoyu Xu, Hong Wang, Ke Wei and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Protein & cell, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Yan BiShanghai Key Laboratory of Maternal and Fetal Medicine, Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.
Jindian HuShanghai Key Laboratory of Maternal and Fetal Medicine, Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.
Tao WuShanghai Key Laboratory of Maternal and Fetal Medicine, Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.ORCID 0000-0001-5640-159X
Zhaohui OuyangShanghai Key Laboratory of Maternal and Fetal Medicine, Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.
Tan LinShanghai Key Laboratory of Maternal and Fetal Medicine, Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.
Jiaxing SunShanghai Key Laboratory of Maternal and Fetal Medicine, Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.
Xinbao ZhangShanghai Key Laboratory of Maternal and Fetal Medicine, Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.
Xiaoyu XuShanghai Key Laboratory of Maternal and Fetal Medicine, Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.
Hong WangShanghai Key Laboratory of Maternal and Fetal Medicine, Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.
Ke WeiInstitute for Regenerative Medicine, State Key Laboratory of Cardiology and Medical Innovation Center, Shanghai Institute of Stem Cell Research and Clinical Translation, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200120, China.ORCID 0000-0001-8542-2146
Shaorong GaoShanghai Key Laboratory of Maternal and Fetal Medicine, Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.ORCID 0000-0003-1041-3928
Yixuan WangShanghai Key Laboratory of Maternal and Fetal Medicine, Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.ORCID 0000-0002-2961-6760

Funding

Chenguang Program of Shanghai Education Development Foundation and Shanghai Municipal Education Commission 24CGA22Fundamental Research Funds for the Central Universities 22120240255Fundamental Research Funds for the Central Universities 22120240319Fundamental Research Funds for the Central Universities 22120250374Ministry of Science and Technology of China 2021YFA11020000Ministry of Science and Technology of China 2022YFC2702200Ministry of Science and Technology of China 2023YFA1800019National Key Research and Development Program of China 2024YFA1107000National Natural Science Foundation of China 32100633National Natural Science Foundation of China 32270850National Natural Science Foundation of China 32370842National Natural Science Foundation of China 32470843National Natural Science Foundation of China 32488101National Natural Science Foundation of China 92168205Peak Disciplines (Type IV) of Institutions of Higher Learning in ShanghaiScience and Technology Commission of Shanghai MunicipalityShuguang Program of the Shanghai Education Development Foundation and Shanghai Municipal Education Commission 22SG20
6 · The paper itself

Abstract

Human naïve pluripotent stem cells (PSCs) hold great promise for embryonic development studies. Existing induction and culture strategies for these cells, heavily dependent on MEK inhibitors, lead to widespread DNA hypomethylation, aberrant imprinting loss, and genomic instability during extended culture. Here, employing high-content analysis alongside a bifluorescence reporter system indicative of human naïve pluripotency, we screened over 1,600 chemicals and identified seven promising candidates. From these, we developed four optimized media-LAY, LADY, LUDY, and LKPY-that effectively induce and sustain PSCs in the naïve state. Notably, cells reset or cultured in these media, especially in the LAY system, demonstrate improved genome-wide DNA methylation status closely resembling that of pre-implantation counterparts, with partially restored imprinting and significantly enhanced genomic stability. Overall, our study contributes advancements to naïve pluripotency induction and long-term maintenance, providing insights for further applications of naïve PSCs.

Indexed as

Cell Culture TechniquesDNA MethylationGenomic InstabilityPluripotent Stem CellsCells, CulturedHumansbifluorescence reporter systemDNA methylationgenomic stabilityhigh-content analysishuman naïve culture system

Identifiers

PMID40578838
PMCPMC12578290

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.