Evidence map›Paper›PMID 40578351›Full record

ArticleStem cell reports2025

Aged human serum induces vascular changes in an isogenic co-culture venule model.

Tracy D Chung, Raleigh M Linville, Nan Zhao, Linus Wang, Zikai Xia, Peter C Searson

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Brain vascular basement membrane: Comparison of human and mouse brain at the transcriptomic and proteomic levels.Matrix biology : journal of the International Society for Matrix Biology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Tracy D ChungInstitute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD, USA; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.
Raleigh M LinvilleInstitute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD, USA.
Nan ZhaoInstitute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD, USA.
Linus WangInstitute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD, USA; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.
Zikai XiaInstitute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD, USA; Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD, USA.
Peter C SearsonInstitute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD, USA; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA; Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD, USA. Electronic address: searson@jhu.edu.

Funding

Tech Core 2U54CA268083 · NCI · JOHNS HOPKINS UNIVERSITY · PI Pei-Hsun wu · 2022 to 2026
$10.2M
Mechanisms of cerebrovascular barrier dysfunction caused by APP and PSEN1 mutations and amyloid beta exposureR01NS106008 · NINDS · JOHNS HOPKINS UNIVERSITY · PI SEARSON, PETER C · 2019 to 2023
$1.9M
Reverse engineering zonation-specific and age-specific iPSC-derived cerebrovascular models based on transcriptomic profiling of the human brainR33HL154252 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI HEIMAN, MYRIAM, SEARSON, PETER C · 2023 to 2025
$1.3M
The combinatorial effects of familial mutations, oxidative stress, and aging on BBB dysfunction in Alzheimer's diseaseF31AG077930 · NIA · JOHNS HOPKINS UNIVERSITY · PI CHUNG, TRACY D. · 2022 to 2024
$117k
NCI NIH HHS U54 CA268083NHLBI NIH HHS R33 HL154252NIA NIH HHS F31 AG077930NINDS NIH HHS R01 NS106008
6 · The paper itself

Abstract

Vascular aging and dysfunction are significant contributors to age-related cardiovascular and neurodegenerative diseases. In particular, aging impacts small vessels, damaging vascular integrity leading to leakage events and inflammation, which can be further exacerbated by environmental factors. Here, we generate and evaluate an isogenic endothelial cell and pericyte venule-like co-culture model of microvasculature under perfusion with male aged human serum over 4 days. Using this model in comparison to male young human serum perfusion controls, we define the molecular and functional changes induced by aging-related circulatory cues, including functional loss of paracellular barrier integrity and modulation of transport of low-density lipoprotein. Additionally, in comparison with endothelial monoculture, we identify critical changes to basement membrane composition and aged-serum-mediated cell cycle shifts with pericyte co-culture. This modular approach reveals key impacts to further our understanding of vascular aging and to leverage in designing therapeutic and preventative approaches.

Indexed as

AgingSerumCoculture TechniquesEndothelial CellsHumansHuman Umbilical Vein Endothelial CellsMalePericytesagingbarrier functionendothelial cellspericytestissue-engineered microvesselsvenule

Identifiers

PMID40578351
PMCPMC12277876

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.