Evidence map›Paper›PMID 40577374›Full record

ArticlePloS one2025

Influence of lighting on sleep behaviour, circadian rhythm and spontaneous blink rate in stabled riding school horses (Equus caballus).

Linda Greening, Eilis Harkin, Panoraia Kyriazopoulou, Zoe Heppelthwaite, Francesca Aragona, John A Browne, Andrew Hemmings, Jane M Williams, Barbara A Murphy

Abstract read
In one paragraph

Article in PloS one, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Linda GreeningEquine Department, Hartpury University, Gloucester, Gloucestershire, United Kingdom.
Eilis HarkinSchool of Agriculture and Food Science, University College Dublin, Belfield, Dublin, Ireland.
Panoraia KyriazopoulouEquilume Ltd, Naas, Co. Kildare, Ireland.ORCID 0000-0001-7029-5973
Zoe HeppelthwaiteRoyal Agricultural University, Cirencester, Gloucestershire, United Kingdom.
Francesca AragonaSchool of Agriculture and Food Science, University College Dublin, Belfield, Dublin, Ireland.
John A BrowneSchool of Agriculture and Food Science, University College Dublin, Belfield, Dublin, Ireland.
Andrew HemmingsRoyal Agricultural University, Cirencester, Gloucestershire, United Kingdom.
Jane M WilliamsEquine Department, Hartpury University, Gloucester, Gloucestershire, United Kingdom.
Barbara A MurphySchool of Agriculture and Food Science, University College Dublin, Belfield, Dublin, Ireland.ORCID 0000-0003-2163-7927

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Modern horse husbandry involves significant time spent indoors, often in suboptimal lighting conditions and with frequent night-time disturbances by humans for management purposes. The aim of this study was to investigate the influence of a customised light-emitting diode (LED) lighting system and a standard fluorescent lighting fixture on equine sleep behaviours, circadian rhythmicity and spontaneous blink rates in horses. Ten riding school horses experienced two stable lighting conditions for four weeks each in a cross-over study running from January to March, 2023. The treatment lighting consisted of an LED system that provided timed, blue-enriched white polychromatic light by day and dim red light at night, and control lighting was a fluorescent tube that was turned on and off manually morning and evening. During week 4 of each experimental period, spontaneous blink rate was recorded twice for 30 min, behaviour of horses in their stables was recorded continuously for 72 h, and hair samples for circadian clock gene analysis were collected at 4-h intervals for 52 h. No differences were detected for total sleep, lateral or sternal recumbency, wakefulness, standing, standing sleep, or spontaneous blink rate (P > 0.05), between lighting conditions. The lighting period (Day versus Night) influenced total sleep (P < 0.01), total recumbency (P < 0.01), wakefulness (P < 0.01), and standing sleep (P < 0.05) in both conditions. For the treatment condition only, higher wakefulness was recorded during Day (P < 0.05). An overall effect of time for clock genes PER2 and DBP was detected (P < 0.01), but there was no effect of treatment, or time by treatment interaction. Cosinor analysis detected significant 24-h rhythmicity for PER2 and DBP (P < 0.01) in both lighting conditions. Results imply that dim red light at night does not negatively impact normal sleep patterns or circadian rhythmicity, and provide evidence supporting further research to better understand the role of blue-enriched LED light at promoting increased wakefulness during daytime in stabled horses.

Indexed as

Behavior, AnimalBlinkingCircadian RhythmLightingSleepAnimalsCircadian ClocksCross-Over StudiesFemaleHorsesMale

Identifiers

PMID40577374
PMCPMC12204570

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.