Evidence map›Paper›PMID 40576853›Full record

ArticleAnnals of biomedical engineering2025

Optical Redox Imaging Predicts Post-Loading Cartilage Mitochondrial Membrane Potential.

Jingyi Wang, Greta E Scheidt, Corinne R Henak

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Annals of biomedical engineering, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Jingyi WangDepartment of Mechanical Engineering, University of Wisconsin-Madison, 3031 Mechanical Engineering Building, 1513 University Ave, Madison, WI, 53706, USA.
Greta E ScheidtDepartment of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA.
Corinne R HenakDepartment of Mechanical Engineering, University of Wisconsin-Madison, 3031 Mechanical Engineering Building, 1513 University Ave, Madison, WI, 53706, USA. chenak@wisc.edu.ORCID http://orcid.org/0000-0002-1525-1350

Funding

Directorate for Engineering CMMI BMMB 2237707
6 · The paper itself

Abstract

purposeDisrupted cellular redox balance is associated with various diseases, including osteoarthritis. Although mitochondrial (MT) membrane potential is a proxy for redox balance, the translational potential of this method is limited by exogenous dye. Therefore, the objective of this study was to predict changes in MT membrane potential in response to mechanical loading using dye-free optical redox imaging (ORI). A secondary objective was to determine the effect of loading on ORI metrics.

methodsFull-thickness porcine cartilage strips were subjected to tensile loading at one of two strain rates (1.00 s

resultsIn the GLMM using MT red/green ratio as the dependent variable, ORI metrics, loading rate, and loading vs. control were significant main effect. GLMMs to predict MT red/green from ORI that retained significant main effects resulted in an average difference between predicted and actual values of 7.07%. When analyzing the effect of loading vs. control, loading rate, and zone on ORI metrics, only loading rate showed significance.

conclusionORI can predict MT membrane potential measured by fluorescent dye and has the possibility to be developed as a clinical tool to evaluate cartilage redox balance.

Indexed as

Cartilage, ArticularMembrane Potential, MitochondrialOptical ImagingAnimalsOxidation-ReductionSwineCartilageMechanobiologyMitochondrial membrane potentialOptical redox imaging

Identifiers

PMID40576853
PMCPMC12390869

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.