Evidence map›Paper›PMID 40576359›Full record

ArticlemBio2025

Combined cellular and proteomics approach suggests differential processing of a native and a foreign vibrio in the sponge

Angela M Marulanda-Gomez, Benjamin Mueller, Kristina Bayer, Mohammad Abukhalaf, Liam Cassidy, Andreas Tholey, Sebastian Fraune, Lucia Pita, Ute Hentschel

Abstract read
In one paragraph

Article in mBio, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Angela M Marulanda-GomezResearch Unit Marine Symbioses, GEOMAR Helmholtz Centre for Ocean Research Kiel, Kiel, Germany.ORCID 0000-0002-9760-0779
Benjamin MuellerDepartment for Freshwater and Marine Ecology, University of Amsterdam, Amsterdam, the Netherlands.ORCID 0000-0001-9335-0437
Kristina BayerResearch Unit Marine Symbioses, GEOMAR Helmholtz Centre for Ocean Research Kiel, Kiel, Germany.ORCID 0000-0003-2789-9805
Mohammad AbukhalafSystematic Proteomics and Bioanalytics, Institute for Experimental Medicine, Christian-Albrechts-Universität Kiel, Kiel, Germany.ORCID 0000-0003-4536-7036
Liam CassidySystematic Proteomics and Bioanalytics, Institute for Experimental Medicine, Christian-Albrechts-Universität Kiel, Kiel, Germany.ORCID 0000-0001-5143-9855
Andreas TholeySystematic Proteomics and Bioanalytics, Institute for Experimental Medicine, Christian-Albrechts-Universität Kiel, Kiel, Germany.ORCID 0000-0002-8687-6817
Sebastian FrauneInstitute of Zoology and Organismic Interactions, Heinrich Heine Universität Düsseldorf, Düsseldorf, Germany.ORCID 0000-0002-6940-9571
Lucia PitaMarine Biogeochemistry, Atmosphere and Climate group, Institut de Ciències del Mar-CSIC, Barcelona, Spain.ORCID 0000-0003-0163-1587
Ute HentschelResearch Unit Marine Symbioses, GEOMAR Helmholtz Centre for Ocean Research Kiel, Kiel, Germany.ORCID 0000-0003-0596-790X

Funding

Deutsche Forschungsgemeinschaft CRC1182-TP B01Fundación Carmen y Severo Ochoa 2021SGR00430Gordon and Betty Moore Foundation GBMF9352HORIZON EUROPE Marie Sklodowska-Curie Actions No 847648HORIZON EUROPE Marie Sklodowska-Curie Actions No 894645'la Caixa' Foundation ('la Caixa') ID 10010434
6 · The paper itself

Abstract

Phagocytosis is a conserved cellular mechanism for food uptake, defense, and animal-microbe interactions in metazoans. How the discrimination and subsequent processing of different microbes in marine invertebrates is facilitated remains largely unknown. Thereto, we combined a recently developed phagocytic assay with proteomics analysis to compare the phagocytic activity of the sponge IMPORTANCE: Metazoans recognize and discriminate between different microbes. In marine invertebrates, the underlying mechanisms of microbial discrimination and immune specificity are, however, not well understood. Phagocytosis is a conserved cellular process from amoeba to humans that facilitates the ingestion and digestion of microbial cells and likely plays a role in this discrimination. To elucidate the molecular and cellular basis of this microbial discrimination, we examined the differential phagocytic processing of a native (i.e., sponge-isolated) and foreign (i.e., sea anemone-isolate)

Indexed as

PhagocytosisPoriferaProteomicsVibrioAnimalsProteomeProteomeanimal-microbe interactionsfluorescence-activated cell sorting (FACS)immune specificityinnate immunityphagocytosisproteomicssponge cellssponge-microbe symbiosisVibrio isolates

Identifiers

PMID40576359
PMCPMC12345144

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.