Evidence map›Paper›PMID 40576153›Full record

ArticleJournal of the American Chemical Society2025

Introduction of Reactive Thiol Handles into Tyrosine-Tagged Proteins through Enzymatic Oxidative Coupling.

Paul Huang, Wendy Cao, Jennifer L Fetzer, Nicholas S Dolan, Matthew B Francis

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of the American Chemical Society, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Precision Labeling of Native Antibodies with Lock Coupling.Journal of the American Chemical Society · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Paul HuangDepartment of Chemistry, University of California, Berkeley, California 94720, United States.ORCID 0000-0002-3520-0032
Wendy CaoDepartment of Chemistry, University of California, Berkeley, California 94720, United States.
Jennifer L FetzerDepartment of Chemistry, University of California, Berkeley, California 94720, United States.ORCID 0009-0009-1059-4598
Nicholas S DolanDepartment of Chemistry, University of California, Berkeley, California 94720, United States.
Matthew B FrancisDepartment of Chemistry, University of California, Berkeley, California 94720, United States.ORCID 0000-0003-2837-2538

Funding

Facile Generation of Protein-Protein Conjugates Using Enzymatic Oxidative Coupling ReactionsR01GM138693 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI FRANCIS, MATTHEW B · 2020 to 2025
$1.5M
NIGMS NIH HHS R01 GM138693
6 · The paper itself

Abstract

Site-specific protein bioconjugation methods have enabled the development of new therapeutics and materials, and further development of existing techniques has expanded the compatible library of protein substrates for bioconjugation. Among these techniques, the enzyme tyrosinase has demonstrated a promising ability to form protein-protein conjugates between exposed tyrosine and cysteine residues. In this work, we observed that the tyrosinase variant from

Indexed as

Monophenol MonooxygenaseSulfhydryl CompoundsTyrosineBacillus megateriumOxidation-ReductionMonophenol MonooxygenaseSulfhydryl CompoundsTyrosine

Identifiers

PMID40576153
PMCPMC12257508

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.