Evidence map›Paper›PMID 40575347›Full record

ArticleRegenerative therapy2025

Sustained release of canine mesenchymal stem/stromal cell-derived extracellular vesicles rescues motor function in rodent spinal cord injury models.

Hidetaka Nishida, Haruhi Matsuura, Karin Yoshizaki, Yusuke Sakaguchi, Takeshi Izawa, Hikaru Sonoda, Kazuya Edamura, Yasuhiko Tabata

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Regenerative therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Extracellular vesicle-based therapeutic strategies for spinal cord injury.Extracellular vesicles and circulating nucleic acids · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Hidetaka NishidaSmall Animal Clinics, Department of Veterinary Medicine, School of Veterinary Medicine, Azabu University, 1-17-71, Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252-5201, Japan.
Haruhi MatsuuraDepartment of Veterinary Surgery, Graduate School of Veterinary Science, Osaka Metropolitan University, 1-58, Rinku Orai Kita, Izumisano, Osaka 598-8531, Japan.
Karin YoshizakiDepartment of Veterinary Surgery, Graduate School of Veterinary Science, Osaka Metropolitan University, 1-58, Rinku Orai Kita, Izumisano, Osaka 598-8531, Japan.
Yusuke SakaguchiSmall Animal Clinics, Department of Veterinary Medicine, School of Veterinary Medicine, Azabu University, 1-17-71, Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252-5201, Japan.
Takeshi IzawaLaboratory of Veterinary Pathology, Graduate School of Veterinary Science, Osaka Metropolitan University, 1-58, Rinku Orai Kita, Izumisano, Osaka 598-8531, Japan.
Hikaru SonodaHakarel Inc, 7-7-18, Saito Asagi, Ibaraki, Osaka 567-0085, Japan.
Kazuya EdamuraLaboratory of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource and Sciences, Nihon University, Fujisawa, Kanagawa 252-0880, Japan.
Yasuhiko TabataCell Biotechnology Group, Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Kyoto University, Kyoto University South Research Bldg, No.1, 53 Kawara-cho Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Extracellular vesicles (EVs) are expected to be a novel promotes in regenerative therapy. EVs derived from canine mesenchymal stromal/stem cells (MSCs) have been shown to play an important role in tissue repair. For clinical application of EV therapy, a major challenge is to find scalable methods for EV collection and to facilitate continuing local EV action. The aim of this study is to develop collection methods for EVs derived from canine MSCs using anion exchange chromatography (AEX), and to determine the therapeutic effects of sustained release of EVs from cationized gelatin hydrogels in rats with spinal cord injury (SCI). Methods: Canine MSC-derived EVs were isolated from the culture supernatant by AEX. Lipopolysaccharide-stimulated BV-2 cells were used to determine the immunomodulatory effect of EVs. Distinct cross-linked cationized gelatin hydrogels were created by thermal dehydration. The amount of EVs captured by and released from hydrogels were measured using ELISA for CD63. The effects of sustained release of EVs on SCI in rats were evaluated via motor function and cavity volume. Results: EVs were collected from the culture supernatant of canine MSCs using AEX. Canine MSC derived EVs collected by AEX significantly reduced the expression levels of IL-1β and IL-6 in BV-2 cells induced by LPS. Sustained release of EVs from cationized hydrogels led to significant improvement of locomotion in SCI rats relative to the control group at 14- and 28-days post-injury. Cavity formation in the spinal cord with sustained release of EVs was significantly less than in the control group. Conclusions: Canine MSC derived EVs cultured in serum-free medium using AEX were successfully collected. Sustained release of canine MSC derived EVs suppresses cavity formation in the spinal cord after SCI and promotes improvement in motor function.

Indexed as

CanineExtracellular vesicleHydrogelMesenchymal stem/stromal cellSpinal cord injurySustained release

Identifiers

PMID40575347
PMCPMC12197974

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.