Evidence map›Paper›PMID 40575300›Full record

ArticleJACS Au2025

Repurposing CDP-Tyvelose 2‑Epimerase Enables a GDP-Fucose-Based Fucosylation Pathway Starting from Sucrose.

Wei Wang, Xiao-Jun Pang, Meng Wang, Yu Tian, Xueyu Wu, Giulia Pergolizzi, Martin Rejzek, Robert A Field, Li Liu, Göran Widmalm and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in JACS Au, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Wei WangGlycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology Nanjing Agricultural University, 1 Weigang, 210095 Nanjing, China.
Xiao-Jun PangGlycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology Nanjing Agricultural University, 1 Weigang, 210095 Nanjing, China.
Meng WangGlycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology Nanjing Agricultural University, 1 Weigang, 210095 Nanjing, China.
Yu TianBeijing Castar Union Technology Co., Ltd., No. 6, Zhongguancun South third Street, Haidian District, 100190 Beijing, China.
Xueyu WuBeijing Castar Union Technology Co., Ltd., No. 6, Zhongguancun South third Street, Haidian District, 100190 Beijing, China.
Giulia PergolizziDepartment of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, U.K.
Martin RejzekDepartment of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, U.K.ORCID https://orcid.org/0000-0002-5091-544X
Robert A FieldDepartment of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, U.K.ORCID https://orcid.org/0000-0001-8574-0275
Li LiuGlycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology Nanjing Agricultural University, 1 Weigang, 210095 Nanjing, China.ORCID https://orcid.org/0000-0002-2178-9237
Göran WidmalmDepartment of Organic Chemistry, Arrhenius Laboratory, Stockholm University, S-106 91 Stockholm, Sweden.ORCID https://orcid.org/0000-0001-8303-4481
Josef VoglmeirGlycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology Nanjing Agricultural University, 1 Weigang, 210095 Nanjing, China.ORCID https://orcid.org/0000-0002-4096-4926

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study introduces a novel enzymatic cascade featuring five recombinant enzymes for the efficient synthesis of fucosylated glycosides, using sucrose exclusively as the sugar donor substrate. In our approach, we employed a sucrose synthase sourced from tomato to generate GDP-glucose from sucrose and GDP. By repurposing CDP-tyvelose 2-epimerase from Salmonella enterica, chosen for its catalytic efficiency from a panel of 27 CDP-tyvelose 2-epimerase candidates, it was possible to epimerize GDP-glucose into GDP-mannose. The subsequent transformation of GDP-d-mannose to GDP-l-fucose was achieved by GDP-mannose 4,6-dehydratase and GDP-4-keto-6-deoxy-d-mannose epimerase/reductase, also derived from S. enterica. In the final stage,

Indexed as

CDP-tyvelose 2-epimeraseenzyme repurposingGDP-fucose biosynthesisrecombinant enzymessucrose utilization

Identifiers

PMID40575300
PMCPMC12188384

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.