Evidence map›Paper›PMID 40573437›Full record

ArticleViruses2025

Re-Emergence of Usutu Virus and Spreading of West Nile Virus Neuroinvasive Infections During the 2024 Transmission Season in Croatia.

Tatjana Vilibić-Čavlek, Ljubo Barbić, Ana Klobučar, Marko Vucelja, Maja Bogdanić, Dario Sabadi, Marko Kutleša, Branimir Gjurašin, Vladimir Stevanović, Marcela Curman Posavec and 10 more

Abstract read
In one paragraph

Article in Viruses, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors.

Tatjana Vilibić-ČavlekDepartment of Virology, Croatian Institute of Public Health, 10000 Zagreb, Croatia.ORCID 0000-0002-1877-5547
Ljubo BarbićDepartment of Microbiology and Infectious Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, 10000 Zagreb, Croatia.ORCID 0000-0002-5170-947X
Ana KlobučarDepartment of Epidemiology, Andrija Štampar Teaching Institute of Public Health, 10000 Zagreb, Croatia.ORCID 0000-0003-3464-6830
Marko VuceljaDepartment of Forest Protection and Wildlife Management, Faculty of Forestry and Wood Technology, University of Zagreb, 10000 Zagreb, Croatia.ORCID 0000-0001-9532-1111
Maja BogdanićDepartment of Virology, Croatian Institute of Public Health, 10000 Zagreb, Croatia.
Dario SabadiDepartment of Infectious Diseases, Clinical Hospital Center Osijek, 31000 Osijek, Croatia.ORCID 0009-0002-8283-6065
Marko KutlešaDepartment of Intensive Care Medicine and Neuroinfectology, University Hospital for Infectious Diseases "Dr. Fran Mihaljević", 10000 Zagreb, Croatia.
Branimir GjurašinDepartment of Intensive Care Medicine and Neuroinfectology, University Hospital for Infectious Diseases "Dr. Fran Mihaljević", 10000 Zagreb, Croatia.
Vladimir StevanovićDepartment of Microbiology and Infectious Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, 10000 Zagreb, Croatia.
Marcela Curman PosavecDepartment of Epidemiology, Andrija Štampar Teaching Institute of Public Health, 10000 Zagreb, Croatia.
Linda BjedovDepartment of Forest Protection and Wildlife Management, Faculty of Forestry and Wood Technology, University of Zagreb, 10000 Zagreb, Croatia.ORCID 0009-0009-5595-8367
Marko BoljfetićDepartment of Forest Protection and Wildlife Management, Faculty of Forestry and Wood Technology, University of Zagreb, 10000 Zagreb, Croatia.
Tonka Jozić-NovincDepartment of Infectious Diseases, General Hospital "Dr. Ivo Pedišić", 44000 Sisak, Croatia.
Robert ŠkaraDepartment of Infectious Diseases, General Hospital "Dr. Ivo Pedišić", 44000 Sisak, Croatia.
Morana TomljenovićDepartment of Epidemiology, Teaching Institute of Public Health of the Primorje-Gorski Kotar County, 51000 Rijeka, Croatia.ORCID 0000-0003-2472-6087
Željka HruškarDepartment of Virology, Croatian Institute of Public Health, 10000 Zagreb, Croatia.ORCID 0000-0002-9078-1773
Mahmoud Al-MuflehDepartment of Infectious Diseases, County Hospital Čakovec, 40000 Čakovec, Croatia.
Tanja Potočnik-HunjadiDepartment of Infectious Diseases, General Hospital Varaždin, 42000 Varaždin, Croatia.
Ivana RončevićPoultry Center, Croatian Veterinary Institute, 10000 Zagreb, Croatia.ORCID 0009-0001-0908-5675
Vladimir SavićPoultry Center, Croatian Veterinary Institute, 10000 Zagreb, Croatia.ORCID 0000-0003-0398-5346

Funding

European Union NextGenerationEU project supported by the Ministry of Science and Education of the Republic of Croatia NPOO 1European Virus Archive goes Global (EVAg) project that has received funding from the Europe-an Union's Horizon 2020 research and innovation programme 653316
6 · The paper itself

Abstract

Neuroinvasive arboviruses such as tick-borne encephalitis virus (TBEV), West Nile virus (WNV), Usutu virus (USUV), and Toscana virus (TOSV) have (re-)emerged with increasing incidence and geographic range. We analyzed the epidemiology of arboviral infections in Croatia during the 2024 transmission season. A total of 154 patients with neuroinvasive diseases (NID), 1596 horses, 69 dead birds, and 7726 mosquitoes were tested. Viral RNA was detected using RT-qPCR. IgM/IgG-specific antibodies were detected using commercial ELISA or IFA, with confirmation of cross-reactive samples by virus neutralization test. RT-qPCR-positive samples were Sanger sequenced. Arboviral etiology was confirmed in 33/21.42% of patients with NID. WNV was most frequently detected (17/11.03%), followed by TBEV (10/6.49%), USUV (5/3.24%), and TOSV (1/0.64%). WNV infections were reported in regions previously known as endemic, while in one continental county, WNV was recorded for the first time. USUV infections re-emerged after a six-year absence. In addition to human cases, acute WNV infections were recorded in 11/395 (2.78%) of horses and two dead crows. WNV IgG seropositivity was detected in 276/1168 (23.63%) and TBEV IgG seropositivity in 68/428 (15.88%) horses. None of the tested mosquito pools were positive for WNV and USUV RNA. Phylogenetic analysis showed the circulation of WNV lineage 2 and Usutu Europe 2 lineage. Climate conditions in 2024 in Croatia were classified as extremely warm, which could, at least in part, impact the quite intense arboviral season. The spreading of flaviviruses in Croatia highlights the need for continuous surveillance in humans, animals, and vectors ("One Health").

Indexed as

FlavivirusFlavivirus InfectionsWest Nile FeverWest Nile virusAdolescentAdultAgedAnimalsAntibodies, ViralBirdsChildCroatiaCulicidaeFemaleHorse DiseasesHorsesAntibodies, ViralImmunoglobulin GImmunoglobulin MRNA, ViralCroatianeuroinvasive infectionstick-borne encephalitis virusUsutu virusWest Nile virus

Identifiers

PMID40573437
PMCPMC12197382

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.