Evidence map›Paper›PMID 40572128›Full record

ArticleMicroorganisms2025

Implementing Wastewater-Based Epidemiology for Long-Read Metagenomic Sequencing of Antimicrobial Resistance in Kampala, Uganda.

William Strike, Temitope O C Faleye, Brian Lubega, Alexus Rockward, Soroosh Torabi, Anni Noble, Mohammad Dehghan Banadaki, James Keck, Henry Mugerwa, Matthew Scotch and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microorganisms, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Co-Occurrence of Resistance to Aminoglycosides and Carbapenems inInternational journal of microbiology · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

William StrikeDepartment of Biomedical Engineering, University of Kentucky, 522 Robotics and Manufacturing Building, Lexington, KY 40506, USA.ORCID 0000-0002-0917-5064
Temitope O C FaleyeBiodesign Center for Environmental Health Engineering, Arizona State University, 1001 S McAllister Ave, Tempe, AZ 85281, USA.ORCID 0000-0001-7706-9493
Brian LubegaJoint Clinical Research Centre, Lubowa Estates, Kampala 759125, Uganda.
Alexus RockwardDepartment of Biomedical Engineering, University of Kentucky, 522 Robotics and Manufacturing Building, Lexington, KY 40506, USA.
Soroosh TorabiDepartment of Mechanical and Aerospace Engineering, University of Kentucky, 151 Ralph G. Anderson Building, Lexington, KY 40506, USA.ORCID 0000-0001-8574-3273
Anni NobleDepartment of Mechanical and Aerospace Engineering, University of Kentucky, 151 Ralph G. Anderson Building, Lexington, KY 40506, USA.ORCID 0009-0009-7378-6466
Mohammad Dehghan BanadakiDepartment of Mechanical and Aerospace Engineering, University of Kentucky, 151 Ralph G. Anderson Building, Lexington, KY 40506, USA.ORCID 0000-0001-6448-3505
James KeckWWAMI School of Medicine, University of Alaska Anchorage, 3211 Providence Dr, Anchorage, AK 99508, USA.
Henry MugerwaJoint Clinical Research Centre, Lubowa Estates, Kampala 759125, Uganda.
Matthew ScotchBiodesign Center for Environmental Health Engineering, Arizona State University, 1001 S McAllister Ave, Tempe, AZ 85281, USA.ORCID 0000-0001-5100-9724
Scott BerryDepartment of Biomedical Engineering, University of Kentucky, 522 Robotics and Manufacturing Building, Lexington, KY 40506, USA.ORCID 0000-0001-7403-9264

Funding

Pilot Project ProgramP30ES026529 · NIEHS · UNIVERSITY OF KENTUCKY · PI John Anthony Bauer · 2017 to 2026
$15.4M
Wastewater Assessment for Coronavirus in Kentucky: Implementing Enhanced Surveillance TechnologyU01DA053903 · NIDA · UNIVERSITY OF KENTUCKY · PI BERRY, SCOTT M · 2021 to 2022
$3.4M
CDC HHS BAA 75D301-20-R-68024NIDA NIH HHS U01 DA053903NIEHS NIH HHS P30 ES026529NIH HHS 1P30ES026529-01NIH HHS 1U01DA053903-01U.S. National Science Foundation 2154934U.S. National Science Foundation 2412446
6 · The paper itself

Abstract

Antimicrobial resistance (AMR) is an emerging global threat that is expanding in many areas of the world. Wastewater-based epidemiology (WBE) is uniquely suited for use in areas of the world where clinical surveillance is limited or logistically slow to identify emerging threats, such as in Sub-Saharan Africa (SSA). Wastewater was analyzed from three urban areas of Kampala, including a local HIV research clinic and two informal settlements. Wastewater extraction was performed using a low-cost, magnetic bead-based protocol that minimizes consumable plastic consumption followed by sequencing on the Oxford Nanopore Technology MinION platform. The majority of the analysis was performed using cloud-based services to identify AMR biomarkers and bacterial pathogens. Assemblies containing AMR pathogens were isolated from all locations. As one example, clinically relevant AMR biomarkers for multiple drug classes were found within

Indexed as

drug-resistant pathogensmetagenomic environmental surveillancewastewater

Identifiers

PMID40572128
PMCPMC12195534

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.