Synergistic Engineering of the Twin-Arginine Translocation (Tat) Pathway and Membrane Capacity Enhances Extracellular Production of Amylosucrase in

Caizhe Wang, Dandan Niu, Yongqing Zhou, Hui Liu, Nokuthula Peace Mchunu, Meng Zhang, Suren Singh, Zhengxiang Wang

Abstract read

In one paragraph

Article in Microorganisms, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Metabolic Engineering ofMicroorganisms · 2025
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Caizhe WangState Key Laboratory of Bio-Based Fiber Materials, Tianjin University of Science & Technology, Tianjin 300457, China.

Dandan NiuState Key Laboratory of Bio-Based Fiber Materials, Tianjin University of Science & Technology, Tianjin 300457, China.

Yongqing ZhouState Key Laboratory of Bio-Based Fiber Materials, Tianjin University of Science & Technology, Tianjin 300457, China.

Hui LiuState Key Laboratory of Bio-Based Fiber Materials, Tianjin University of Science & Technology, Tianjin 300457, China.

Nokuthula Peace MchunuNational Research Foundation, P.O. Box 2600, Pretoria 0001, South Africa.

Meng ZhangState Key Laboratory of Bio-Based Fiber Materials, Tianjin University of Science & Technology, Tianjin 300457, China.

Suren SinghDepartment of Biotechnology and Food Science, Faculty of Applied Sciences, Durban University of Technology, P.O. Box 1334, Durban 4001, South Africa.

Zhengxiang WangState Key Laboratory of Bio-Based Fiber Materials, Tianjin University of Science & Technology, Tianjin 300457, China.

Funding

Intergovernmental International Scientific and Technological In-novation Cooperation Program, MOST, China 2021YFE0106200Tianjin Outstanding Talent Program JC20200309

6 · The paper itself

Abstract

Amylosucrase (AS) is a highly versatile enzyme with significant potential for industrial applications, including functional food production and glycosylation of bioactive compounds. However, its large-scale production is hampered by low secretion efficiency in microbial hosts. This study focuses on engineering the twin-arginine translocation (TAT) pathway and optimizing membrane resource allocation in

Indexed as

amylosucraseBacillus licheniformisenzyme productionextracellular enzyme secretionmembrane resource engineeringtwin-arginine translocation (TAT) pathway

Identifiers

PMID40572067

DOI10.3390/microorganisms13061179

PMCPMC12195555

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.