Evidence map›Paper›PMID 40571783›Full record

ArticleExperimental brain research2025

BMSCs-derived exosomal Egr2 inhibited OGD/R-induced neuronal cell injury through the RNF8/DAPK1 axis in ischemic stroke.

Rongjun Xiao, Qingsong Wang, Jun Peng, Xiqi Hu, Min Chen, Ying Xia

Abstract read
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In one paragraph

Article in Experimental brain research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Rongjun XiaoDepartment of Neurosurgery, Central South University Xiangya School of Medicine Affiliated Haikou Hospital, No.1, Liuxi Road, Haidian Island, Haikou, 570208, Hainan Province, China.
Qingsong WangDepartment of Neurosurgery, Central South University Xiangya School of Medicine Affiliated Haikou Hospital, No.1, Liuxi Road, Haidian Island, Haikou, 570208, Hainan Province, China.
Jun PengDepartment of Neurosurgery, Central South University Xiangya School of Medicine Affiliated Haikou Hospital, No.1, Liuxi Road, Haidian Island, Haikou, 570208, Hainan Province, China.
Xiqi HuDepartment of Neurosurgery, Central South University Xiangya School of Medicine Affiliated Haikou Hospital, No.1, Liuxi Road, Haidian Island, Haikou, 570208, Hainan Province, China.
Min ChenDepartment of Neurosurgery, Central South University Xiangya School of Medicine Affiliated Haikou Hospital, No.1, Liuxi Road, Haidian Island, Haikou, 570208, Hainan Province, China.
Ying XiaDepartment of Neurosurgery, Central South University Xiangya School of Medicine Affiliated Haikou Hospital, No.1, Liuxi Road, Haidian Island, Haikou, 570208, Hainan Province, China. xy1125xy@163.com.

Funding

BMSC Derived Exosomal Egr2 Ameliorates Ischemic Stroke by Directly Upregulating SIRT6 to Suppress Notch Signaling 823QN369
6 · The paper itself

Abstract

Bone marrow-derived mesenchymal stem cells (BMSCs) can facilitate functional rehabilitation after ischemic stroke (IS) by secreting exosomes. This study aimed to elucidate the specific mechanism of action of BMSC-derived exosomal Egr2 in OGD/R-induced neuronal cell damage. Exosomes were isolated from BMSCs. OGD/R-treated N2a cells were used for in vitro experiments. CCK8 and flow cytometry analysis were applied to measure cell viability, apoptosis, and BMSCs surface markers. Protein levels were analyzed using western blotting. The binding sites of Egr2 on the RNF8 promoter were predicted using the JASPAR database and verified using ChIP and dual-luciferase reporter assays. Co-IP was used to validate the relationship between RNF8 and DAPK1. Cellular localization of RNF8 and DRPK1 was confirmed by immunofluorescence staining. Egr2 was enriched in BMSC-derived exosomes. Exosomal Egr2 isolated from BMSCs increased the viability and reduced the apoptosis of OGD/R-treated N2a cells. However, these effects were abrogated by Egr2 knockdown. Egr2 activated RNF8 by binding to its promoter. In addition, RNF8 negatively regulated DAPK1 by promoting DAPK1 ubiquitination to alleviate OGD/R-stimulated neuronal cell damage. RNF8 overexpression or DAPK1 knockdown reversed OGD/R and Egr2 knockdown in BMSC co-treated with neuronal cell injury. BMSCs-derived exosomal Egr2 relieved OGD/R-treated neuronal cell injury by regulating the RNF8/DAPK1 axis.

Indexed as

Death-Associated Protein KinasesEarly Growth Response Protein 2ExosomesIschemic StrokeMesenchymal Stem CellsNeuronsUbiquitin-Protein LigasesAnimalsApoptosisCell HypoxiaCell SurvivalMaleMiceDeath-Associated Protein KinasesEarly Growth Response Protein 2Egr2 protein, mouseUbiquitin-Protein LigasesBMSCsDAPK1Egr2ExosomeIschemic strokeRNF8

Identifiers

PMID40571783

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.