ArticleJournal of advanced research2026
Designing an apoptosis reporter by mutagenesis-based insertion of caspase-3 cleavage motif into green fluorescence protein.
Article in Journal of advanced research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
introductionApoptosis is an essential process for organisms and animal development, and its dysregulation is related to the progression of several diseases. Therefore, methods to detect apoptosis are necessary for mechanistic research and drug development. To overcome the disadvantages of traditional methods for detecting apoptosis, a variety of imaging strategies based on enzyme-mediated fluorescence activation have been developed.
objectivesThis study aims to design a novel strategy for apoptosis fluorescent reporters which are inactivated by caspase-3.
methodsFour candidate EGFP mutations containing DEVD-similar sequences were selected by their structural positions and hydrophilicities, and an EGFP mutant was chosen by investigating fluorescence expression. This EGFP mutant was stably expressed in various cells using the safe harbor locus. To verify our apoptosis reporter system, protein levels, colocalization, and intensity were investigated in apoptosis-induced EGFP mutant-expressing cells.
resultsThe fluorescence intensity of the mutant EGFP was decreased in a time- and concentration-dependent manner by staurosporine and H
conclusionOur method does not require additional peptides, which makes it easily adaptable to other systems. And, our apoptosis reporter may be useful in a variety of research fields as well as drug screening.
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