ArticleCell and tissue banking2025
Effects of calcium-sensitive receptors on chondrogenic differentiation of rat bone marrow mesenchymal stem cells.
Article in Cell and tissue banking, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed.
- Calcium-sensing receptor: a potential target for liver health and diseases.Journal of translational medicine · 2025Review
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Authors and funding
9 authors.
Funding
Abstract
The purpose of this study was to investigate the role of Calcium-Sensing Receptor in the chondrogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and to provide a new target for cartilage defect repair. BMSCs were cultured in vitro, and cultured in the complete culture medium with gradient concentration of calcium sensitive receptor inhibitor and activator, and the optimum dose was selected by CCK-8 experiment. The experiment was divided into four groups. After 7, 14 and 21 days of intervention, the intracellular calcium concentration was detected by laser confocal microscope, the differentiation of cartilage was detected by toluidine blue staining, and the expression of cartilage marker proteins (Col- II, Agg and Sox9) was detected by immunocytochemical staining and Western Blot. The CCK-8 assay results showed that the optimal concentrations of Gd and NPS were 300 μM and 10 μM, respectively. After 7, 14, and 21 days of culture, intracellular calcium fluorescence decreased, with notably higher cartilage differentiation in the NPS inhibitor group. Col-II, Agg and Sox9 chondrocyte marker proteins increased with culture time in all groups, with significantly higher levels in the inhibitor group compared to others, followed by the cartilage induction solution group, and then the activator group. Inhibition of calcium sensitive receptors can promote chondrogenic differentiation of rat BMSCs by regulating Sox9, affecting Col- II and Agg.
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Registered trials
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