Generation of Interferon-γ-Expressing Single-Cycle Viral Hemorrhagic Septicemia Virus (rVHSV-A-IFNγ-ΔG) and Potential as a Vaccine in Olive Flounder (Paralichthys olivaceus).

So Yeon Kim, Jun Soung Kwak, Min Sun Kim, Mariem Bessaid, Kyung Min Lee, Ki Hong Kim

Abstract read

In one paragraph

Article in Marine biotechnology (New York, N.Y.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

So Yeon KimDepartment of Biological Sciences, Kongju National University, Gongju, 32588, South Korea.

Jun Soung KwakDepartment of Aquatic Life Medicine, Kongju National University, Gongju, 32588, South Korea.

Min Sun KimDepartment of Biological Sciences, Kongju National University, Gongju, 32588, South Korea.

Mariem BessaidDepartment of Aquatic Life Medicine, Pukyong National University, Busan, 48513, South Korea.

Kyung Min LeeDepartment of Aquatic Life Medicine, Pukyong National University, Busan, 48513, South Korea.

Ki Hong KimDepartment of Aquatic Life Medicine, Pukyong National University, Busan, 48513, South Korea. khkim@pknu.ac.kr.

Funding

Ministry of Science and ICT, South Korea RS-2024-00405986

6 · The paper itself

Abstract

Single-cycle viruses generated by the deletion of gene(s) essential for viral replication in the genome can be a way to enhance the safety of attenuated virus-based vaccines because single-cycle viruses can infect cells only once and cannot produce infective viral particles. In the present study, not only to guarantee safety as a prophylactic vaccine but also to enhance the protective efficacy, a single-cycle viral hemorrhagic septicemia virus (VHSV) lacking the G gene and containing olive flounder (Paralichthys olivaceus) interferon-γ (IFNγ) ORF between N and P genes of the viral genome (rVHSV-A-IFNγ-ΔG) was rescued using reverse genetic technology, and its protective potential was evaluated through the immunization of olive flounder. In the challenge experiment, the cumulative mortality in the control group reached 80%, while groups of fish immunized with various doses of rVHSV-A-IFNγ-ΔG (ranging from 1 × 10

Indexed as

Fish DiseasesFlounderHemorrhagic Septicemia, ViralInterferon-gammaNovirhabdovirusViral VaccinesAnimalsVaccines, AttenuatedInterferon-gammaVaccines, AttenuatedViral VaccinesInterferon-γOlive flounderSingle-cycle rVHSVVaccine efficacy

Identifiers

PMID40569449

DOI10.1007/s10126-025-10483-4

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.