Evidence map›Paper›PMID 40569389›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2025

Testicular somatic and germ cell maturation during rhesus macaque development.

Enrique Sosa, Sissy E Wamaitha, Fei-Man Hsu, Mary Jasmine D Lara, Kiana Oyama, Maggie Custer, Melinda Murphy, Jon D Hennebold, Young Sun Hwang, Amander T Clark

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Enrique SosaDepartment of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA 90095.
Sissy E WamaithaDepartment of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA 90095.ORCID 0000-0001-6245-6255
Fei-Man HsuDepartment of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA 90095.
Mary Jasmine D LaraDepartment of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA 90095.ORCID 0009-0008-3737-3907
Kiana OyamaDivision of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Beaverton, OR 97006.
Maggie CusterDivision of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Beaverton, OR 97006.ORCID 0009-0001-8781-4802
Melinda MurphyDivision of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Beaverton, OR 97006.
Jon D HenneboldDivision of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Beaverton, OR 97006.ORCID 0000-0002-6760-3733
Young Sun HwangDepartment of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA 90095.ORCID 0000-0002-6443-9579
Amander T ClarkDepartment of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA 90095.ORCID 0000-0003-2483-3982

Funding

Upgrade of confocal microscopy at the Oregon National Primate Research CenterP51OD011092 · OD · OREGON HEALTH & SCIENCE UNIVERSITY · PI Bonnie J. Nagel · 2012 to 2026
$203.9M
UCLA Clinical Translational Science InstituteUL1TR001881 · NCATS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ARLEEN F. BROWN, ARASH NAEIM · 2016 to 2026
$118.1M
Towards a preclinical model for overcoming infertility with induced pluripotent stem cellsR01HD098278 · NICHD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CLARK, AMANDER · 2019 to 2023
$3.0M
HHS | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) HD098278HHS | NIH (NIH) OD011092NCATS NIH HHS UL1 TR001881NICHD NIH HHS R01 HD098278NIH HHS P51 OD011092UC | UCLA | Clinical and Translational Science Institute, University of California, Los Angeles (CTSI) UL1TR001881
6 · The paper itself

Abstract

The formation of bilateral testes in animals is critical for puberty, reproductive capacity, and testosterone production across the life course. In humans, testis development begins in embryonic life in the first trimester, with considerable effort focused on the cell and developmental events associated with testis cell specification, leaving limited knowledge on testicular organogenesis during the second and third trimesters. To fill this knowledge gap, we evaluated testicular cell maturation at weeks 5 (W5), W6, W8, W15, and W19 postconception using a rhesus macaque model. Our data identify a major transcriptional change in the somatic cells of the testis (Sertoli cells, interstitial cells and fetal Leydig cells) between W8 and W15, and this is associated with the maturation of seminiferous cords and maturation of PGCs into fetal spermatogonia. Through this work, we identified cellular changes and differential protein expression between W5 and W19 that can be used to holistically define testis development across the time course of embryonic and fetal life. This study provides important insights necessary to recreate the testicular niche from stem cells for biomedical research.

Indexed as

Germ CellsTestisAnimalsCell DifferentiationGene Expression Regulation, DevelopmentalLeydig CellsMacaca mulattaMaleOrganogenesisSertoli CellsSpermatogoniagonadal developmentrhesus macaquesingle-cell RNA-seqtesticular maturation

Identifiers

PMID40569389
PMCPMC12232671

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.