ArticleMolecular biology reports2025
Improved genomic DNA extraction from citrus species using a modified CTAB method.
Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed.
- FaNAC29: a novel gene positively regulating strawberry powdery mildew resistance.The New phytologist · 2026Article
- Mechanism of Terpene Formation inInternational journal of molecular sciences · 2026Article
- Characterization of mitochondrial genomes from three medicinal species of rutaceae and comparative analysis within the family: insights into evolution.BMC genomics · 2026Article
- Analysis of Differential Metabolites in Citrus Leaves Infected with Huanglongbing and Screening of Biomarkers for Early Detection.Molecules (Basel, Switzerland) · 2026Article
- Mining and Analysis of Salt Tolerance Genes in Maize at the Seedling Stage.Current issues in molecular biology · 2026Article
- A novelFrontiers in plant science · 2026Article
- The complete chloroplast genome sequence ofMitochondrial DNA. Part B, Resources · 2026Article
- Article
- Genetic diversity analysis of angled luffa germplasm resources based on phenotypic traits and resequencing data.Frontiers in plant science · 2026Article
- Characterization of White Frost on Exocarpium Citri Grandis: Flavonoid Crystallization Enhances Anti-Inflammatory Activities.Foods (Basel, Switzerland) · 2025Article
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4 authors.
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Abstract
backgroundCitrus species are recalcitrant to DNA extraction due to high levels of polysaccharides, polyphenols, and secondary metabolites that interfere with DNA yield and purity. This study presents an optimized cetyltrimethylammonium bromide (CTAB)-based protocol for efficient genomic DNA isolation from citrus leaves.
methodsLeaf tissues were ground in liquid nitrogen and extracted using a CTAB buffer supplemented with 2% β-mercaptoethanol and 2% polyvinylpyrrolidone. Contaminants were removed by two rounds of phenol: chloroform: isoamyl alcohol (25:24:1) extraction. RNase A treatment was applied, followed by DNA precipitation using cold isopropanol. DNA quality was assessed by A260/280 absorbance ratios and agarose gel electrophoresis. Statistical comparisons were made using the Mann-Whitney U test, Cohen's d, and bootstrap resampling.
resultsThe modified protocol yielded high-molecular-weight DNA with A260/280 ratios ranging from 1.78 to 1.98, indicating high purity. Electrophoresis confirmed intact DNA with minimal RNA contamination. DNA yield and band intensity were significantly higher than with the conventional method (p < 0.001, Cohen's d = -2.10). A positive correlation (r = 0.23) was observed between yield and band intensity. Bootstrap confidence intervals for mean band intensity were 0.11-0.20 (conventional) and 2.91-4.84 (modified).
conclusionThe optimized CTAB protocol provides a simple, cost-effective, and reproducible method for high-quality genomic DNA extraction from citrus, suitable for PCR, genotyping, and sequencing applications.
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