Evidence map›Paper›PMID 40568302›Full record

ArticleISME communications2025

Systematic evaluation of protein extraction for metaproteomic analysis of marine sediment with high clay content.

Anne Ostrzinski, Benoit J Kunath, André Rodrigues Soares, Cédric C Laczny, Rashi Halder, Jens Kallmeyer, Rolando di Primio, Paul Wilmes, Alexander J Probst, Anke Trautwein-Schult and 1 more

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Article in ISME communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Anne OstrzinskiDepartment of Microbial Proteomics, Institute of Microbiology, University of Greifswald, 17489 Greifswald, Germany.
Benoit J KunathLuxembourg Centre for Systems Biomedicine, University of Luxembourg, 4362 Esch-sur-Alzette, Luxembourg.
André Rodrigues SoaresDepartment of Environmental Metagenomics, Research Center One Health Ruhr, University Alliance Ruhr, Faculty of Chemistry, University of Duisburg-Essen, 45141 Essen, Germany.
Cédric C LacznyLuxembourg Centre for Systems Biomedicine, University of Luxembourg, 4362 Esch-sur-Alzette, Luxembourg.
Rashi HalderLuxembourg Centre for Systems Biomedicine, University of Luxembourg, 4362 Esch-sur-Alzette, Luxembourg.
Jens KallmeyerSubsurface Geochemistry, Section Geomicrobiology, GFZ Helmholtz Centre for Geoscience, 14473 Potsdam, Germany.
Rolando di PrimioExploration Manager Play Analysis and Access, Aker BP, 1366 Lysaker, Norway.
Paul WilmesLuxembourg Centre for Systems Biomedicine, University of Luxembourg, 4362 Esch-sur-Alzette, Luxembourg.
Alexander J ProbstDepartment of Environmental Metagenomics, Research Center One Health Ruhr, University Alliance Ruhr, Faculty of Chemistry, University of Duisburg-Essen, 45141 Essen, Germany.
Anke Trautwein-SchultDepartment of Microbial Proteomics, Institute of Microbiology, University of Greifswald, 17489 Greifswald, Germany.
Dörte BecherDepartment of Microbial Proteomics, Institute of Microbiology, University of Greifswald, 17489 Greifswald, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Marine sediments harbor extremely diverse microbial communities that contribute to global biodiversity and play an essential role in the functioning of ecosystems. However, the metaproteome of marine sediments is still poorly understood. The extraction of proteins from environmental samples is still a challenge, especially from marine sediments, due to the complexity of the matrix. Therefore, methods for protein extraction from marine sediments need to be improved. To develop an effective workflow for protein extraction for clayey sediments, we compared, combined and enhanced different protein extraction methods. The workflow presented here includes blocking of protein binding sites on sediment particles with high concentrations of amino acids, effective cell lysis by ultrasonic capture, electro-elution, and simultaneous fractionation of proteins. To test the protocol's efficacy, we added

Indexed as

marine sedimentMetaproteomicsprotein extraction

Identifiers

PMID40568302
PMCPMC12192440

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.