Evidence map›Paper›PMID 40568087›Full record

ArticlebioRxiv : the preprint server for biology2025

The mechanism of ribosomal recruitment during translation initiation on Type 2 IRESs.

Sayan Bhattacharjee, Irina S Abaeva, Zuben P Brown, Yani Arhab, Hengameh Fallah, Christopher U T Hellen, Joachim Frank, Tatyana V Pestova

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Ins and outs of IRES elements: function and significance.Biochemical Society transactions · 2025
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Sayan BhattacharjeeDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
Irina S AbaevaDepartment of Cell Biology, SUNY Downstate Health Sciences University, Brooklyn, NY 11203, USA.
Zuben P BrownDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
Yani ArhabDepartment of Cell Biology, SUNY Downstate Health Sciences University, Brooklyn, NY 11203, USA.ORCID 0000-0002-6165-3203
Hengameh FallahDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
Christopher U T HellenDepartment of Cell Biology, SUNY Downstate Health Sciences University, Brooklyn, NY 11203, USA.
Joachim FrankDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
Tatyana V PestovaDepartment of Cell Biology, SUNY Downstate Health Sciences University, Brooklyn, NY 11203, USA.

Funding

STRUCTURE ANALYSIS OF MACROMOLECULAR ASSEMBLIESR01GM029169 · NIGMS · WADSWORTH CENTER · PI FRANK, JOACHIM · 1985 to 2019
$6.1M
Mechanisms of eukaryotic translation and ribosome-associated mRNA surveillance and protein quality controlR35GM122602 · NIGMS · SUNY DOWNSTATE MEDICAL CENTER · PI TATYANA V PESTOVA · 2017 to 2026
$4.8M
Structural Studies of Macromolecular Assemblies Using Cryo-EMR35GM139453 · NIGMS · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI FRANK, JOACHIM · 2021 to 2025
$3.9M
Alternative mechanisms of different stages in eukaryotic translationR01GM097014 · NIGMS · SUNY DOWNSTATE MEDICAL CENTER · PI HELLEN, CHRISTOPHER ULRICH TRISTRAM · 2012 to 2022
$2.6M
IRES-mediated initiation of picornavirus translationR21AI188505 · NIAID · SUNY DOWNSTATE MEDICAL CENTER · PI CHRISTOPHER Ulrich Tristram HELLEN · 2025 to 2026
$441k
NIAID NIH HHS R21 AI188505NIGMS NIH HHS R01 GM029169NIGMS NIH HHS R01 GM097014NIGMS NIH HHS R35 GM122602NIGMS NIH HHS R35 GM139453
6 · The paper itself

Abstract

The encephalomyocarditis virus (EMCV) IRES and other Type 2 IRESs comprise domains H-L and specifically interact with eIF4G/eIF4A through their essential JK domain. However, the JK domain is not sufficient for IRES function, which also requires the preceding domain I of unknown function. To identify interactions that drive ribosomal recruitment of eIF4G/eIF4A-bound Type 2 IRESs, we determined the cryo-EM structure of 48S initiation complexes formed on the EMCV IRES. It revealed that the apical domain I cloverleaf contacts ribosomal proteins uS13 and uS19 via its Id subdomain and that the essential GNRA tetraloop in subdomain Ic interacts directly with the TψC domain of initiator tRNA. Functional assays supported the exceptional role of these interactions for initiation on this IRES. The strong conservation of primary and secondary structures of the apex of domain I among Type 2 IRESs suggests that the reported interactions are a common essential feature of them all.

Indexed as

cryo-EMEMCV IRESGNRA tetraloopinitiationpicornavirusribosomeType 2 IRESuS13uS19

Identifiers

PMID40568087
PMCPMC12191231

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.