Evidence map›Paper›PMID 40568086›Full record

ArticlebioRxiv : the preprint server for biology2025

Computationally designed stem-epitope mimetics elicit broadly reactive antibodies.

Sarah Wehrle, Andreas Scheck, Laura Reusch, Flavio Matassoli, Sandrine Georgeon, Karla M Castro, Johannes Cramer, Wayne Harshbarger, Stéphane Rosset, Sarah F Andrews and 11 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors.

Sarah WehrleInstitute of Bioengineering, École Polytechnique Fédérale de Lausanne, Lausanne CH-1015, Switzerland.
Andreas ScheckInstitute of Bioengineering, École Polytechnique Fédérale de Lausanne, Lausanne CH-1015, Switzerland.ORCID 0000-0002-2123-6499
Laura ReuschDepartment of Microbiology and Immunology, University of Gothenburg, Gothenburg, Sweden.
Flavio MatassoliVaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
Sandrine GeorgeonInstitute of Bioengineering, École Polytechnique Fédérale de Lausanne, Lausanne CH-1015, Switzerland.
Karla M CastroInstitute of Bioengineering, École Polytechnique Fédérale de Lausanne, Lausanne CH-1015, Switzerland.ORCID 0000-0002-7753-6060
Johannes CramerInstitute of Virology, Hannover Medical School, Hannover 30625, Germany.
Wayne HarshbargerGSK, Rockville, MD, USA.
Stéphane RossetInstitute of Bioengineering, École Polytechnique Fédérale de Lausanne, Lausanne CH-1015, Switzerland.
Sarah F AndrewsVaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
Karin SchönDepartment of Microbiology and Immunology, University of Gothenburg, Gothenburg, Sweden.
Badiaa BouzyaGSK, Rixensart, Belgium.
Ronan RouxelGSK, Rixensart, Belgium.
Normand BlaisGSK, Rixensart, Belgium.
Enrico MalitoGSK, Rockville, MD, USA.
Adrian McDermottVaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
Thomas KreyInstitute of Virology, Hannover Medical School, Hannover 30625, Germany.
Corey P MallettGSK, Rockville, MD, USA.
Ventzislav VassilevGSK, Rixensart, Belgium.
Davide AngelettiDepartment of Microbiology and Immunology, University of Gothenburg, Gothenburg, Sweden.ORCID 0000-0002-5256-1972
Bruno E CorreiaInstitute of Bioengineering, École Polytechnique Fédérale de Lausanne, Lausanne CH-1015, Switzerland.ORCID 0000-0002-7377-8636

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Broad protection against diverse influenza viruses can be conferred by broadly neutralizing antibodies (bnAbs) targeting a conserved site on the hemagglutinin (HA) stem domain. However, the low immunogenicity of this antigenic region hinders the robust induction of such antibodies. Here, we showcase a structure-based immunogen design strategy focusing on the surface mimicry of antigenic sites. By leveraging the structural definition of a stem epitope, we apply computational protein design to develop epitope mimetics to focus the immune response against this site of viral vulnerability. The structurally complex antigenic site is displayed on heterologous protein scaffolds, retaining excellent binding towards known HA stem-specific bnAbs. Our epitope-mimetic induces stem-specific antibodies against highly divergent group 1 and 2 subtypes. The results provide a general framework for the design of novel immunogens eliciting focused immune responses which may be a valuable tool in the development of effective vaccine candidates against other variable pathogens.

Indexed as

broadly neutralizing antibodiescomputational protein designepitope-focused vaccineshemagglutinininfluenza virussurface mimicry

Identifiers

PMID40568086
PMCPMC12191098

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.