Evidence map›Paper›PMID 40567517›Full record

ArticleTransfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie2025

Irradiation with 265-nm Ultraviolet Light-Emitting Diodes to Plasma: Alterations of Hemostatic Parameters and von Willebrand Factor.

Tomoya Hayashi, Yoshihiro Fujimura, Yoshihiko Sakurai, Mikako Masaki, Akihiro Fuchizaki, Kumiko Oguma, Kazuya Hosokawa, Yuichi Mishima, Mitsunobu Tanaka, Kazuta Yasui and 5 more

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In one paragraph

Article in Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Tomoya HayashiJapanese Red Cross Kinki Block Blood Center, Ibaraki, Japan.
Yoshihiro FujimuraJapanese Red Cross Kinki Block Blood Center, Ibaraki, Japan.
Yoshihiko SakuraiJapanese Red Cross Kinki Block Blood Center, Ibaraki, Japan.
Mikako MasakiJapanese Red Cross Kinki Block Blood Center, Ibaraki, Japan.
Akihiro FuchizakiJapanese Red Cross Kinki Block Blood Center, Ibaraki, Japan.
Kumiko OgumaDepartment of Urban Engineering, Graduate School of Engineering, The University of Tokyo, Bunkyo-ku, Japan.
Kazuya HosokawaResearch Center, Fujimori Kogyo Co., Ltd., Yokohama, Japan.
Yuichi MishimaJapanese Red Cross Kinki Block Blood Center, Ibaraki, Japan.
Mitsunobu TanakaDepartment of Preventive Medicine and Public Health National Defense Medical College, Tokorozawa, Japan.
Kazuta YasuiJapanese Red Cross Kinki Block Blood Center, Ibaraki, Japan.
Takafumi KimuraJapanese Red Cross Kinki Block Blood Center, Ibaraki, Japan.
Yoshihiko TaniJapanese Red Cross Central Blood Institute, Japanese Red Cross, Koto-ku, Japan.
Fumiya HirayamaJapanese Red Cross Osaka Red Cross Blood Center, Joto-ku, Japan.
Koki TakahashiJapanese Red Cross Blood Service Headquarters, Japanese Red Cross, Minato-ku, Japan.
Yoshihiro TakiharaJapanese Red Cross Kinki Block Blood Center, Ibaraki, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Among pathogen inactivation methods for blood products, an ultraviolet C (UVC) irradiation has been attracting attention. We previously reported that 265 nm UV-LED (UVC-LED) effectively prevents bacterial infection. However, the effect of UVC-LED on plasma was not considered. This study aimed to explore the effects of high-dose UVC-LED irradiation, required for viral inactivation, on plasma hemostatic potential. Methods: After irradiation of plasma with high-dose UVC-LED for 60 min, the activities of coagulation factors and coagulation inhibitors and von Willebrand factor (VWF) antigen levels were determined. VWF multimer assay and global hemostatic analysis using reconstituted blood under flow were also conducted. Results: UVC-LED irradiation prolonged PT and activated partial thromboplastin time in a fluence-dependent manner. Fibrinogen levels and factors V and XI activities decreased to 40-47% of pre-irradiation levels. VWF multimer analysis revealed a striking reduction in high-molecular-weight VWF multimers, irrespective to ADAMTS13 activity, while VWF antigen levels showed a slight decrease. Electrophoresis under reducing conditions indicated minimal changes in the 250 kDa-VWF subunit band, suggesting that UVC-LED irradiation disrupts inter-subunit disulfide bonds in VWF multimers. Global hemostatic analysis using reconstituted blood with irradiated plasma showed a marked prolongation in primary hemostasis, indicating impaired platelet adhesion to collagen, reflecting reduced high-molecular-weight VWF multimers. Conclusions: We demonstrated for the first time that a high-dose UVC irradiation to plasma cleaves the inter-subunit disulfide bonds of VWF multimers and reduces the molecular size. In plasma products, the balance between maintaining hemostatic activity and inactivating various pathogens by UVC irradiation is a challenge for the future.

Indexed as

265-nm ultraviolet light-emitting diodeCoagulant factorGlobal hemostasisHigh molecular weight multimerVon Willebrand factor

Identifiers

PMID40567517
PMCPMC12187161

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