Evidence map›Paper›PMID 40566504›Full record

ArticleLife (Basel, Switzerland)2025

One-Year Monitoring of the Evolution of SARS-CoV-2 Omicron Subvariants Through Wastewater Analysis (Central Italy, August 2023-July 2024).

Alessandra Nappo, Maya Petricciuolo, Giulia Berno, Agnese Carnevali, Cesare Ernesto Maria Gruber, Giulia Bicchieraro, Roberta Spaccapelo, Martina Rueca, Fabrizio Carletti, Pietro Giorgio Spezia and 7 more

Abstract read
In one paragraph

Article in Life (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Alessandra NappoLaboratory of Virology, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, 00149 Rome, Italy.ORCID 0000-0002-3036-7597
Maya PetricciuoloLaboratory of Applied and Environmental Microbiology, Department of Chemistry, Biology and Biotechnology, University of Perugia, 06122 Perugia, Italy.ORCID 0000-0003-2837-4517
Giulia BernoLaboratory of Virology, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, 00149 Rome, Italy.ORCID 0000-0003-0331-2627
Agnese CarnevaliLaboratory of Applied and Environmental Microbiology, Department of Chemistry, Biology and Biotechnology, University of Perugia, 06122 Perugia, Italy.
Cesare Ernesto Maria GruberLaboratory of Virology, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, 00149 Rome, Italy.ORCID 0000-0002-8831-3380
Giulia BicchieraroDepartment of Medicine and Surgery and Center of Functional Genomics (C.U.R.Ge.F), University of Perugia, 06132 Perugia, Italy.
Roberta SpaccapeloDepartment of Medicine and Surgery and Center of Functional Genomics (C.U.R.Ge.F), University of Perugia, 06132 Perugia, Italy.ORCID 0000-0003-2100-6172
Martina RuecaLaboratory of Virology, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, 00149 Rome, Italy.ORCID 0000-0001-5162-046X
Fabrizio CarlettiLaboratory of Virology, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, 00149 Rome, Italy.ORCID 0000-0003-1952-5117
Pietro Giorgio SpeziaLaboratory of Virology, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, 00149 Rome, Italy.ORCID 0000-0002-6743-014X
Carolina VeneriNational Center for Water Safety (CeNSia), Istituto Superiore di Sanità, 00161 Rome, Italy.
Giuseppina La RosaNational Center for Water Safety (CeNSia), Istituto Superiore di Sanità, 00161 Rome, Italy.ORCID 0000-0002-2657-100X
Elisabetta SuffrediniDepartment of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità, 00161 Rome, Italy.ORCID 0000-0001-7605-0539
Daniele FocosiNorth-Western Tuscany Blood Bank, Pisa University Hospital, 56124 Pisa, Italy.ORCID 0000-0001-8811-195X
Giovanni ChillemiBioinformatics Research Unit in Infectious Diseases, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, 00149 Rome, Italy.ORCID 0000-0003-3901-6926
Ermanno FedericiLaboratory of Applied and Environmental Microbiology, Department of Chemistry, Biology and Biotechnology, University of Perugia, 06122 Perugia, Italy.ORCID 0000-0003-4983-1967
Fabrizio MaggiLaboratory of Virology, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, 00149 Rome, Italy.ORCID 0000-0001-7489-5271

Funding

Ministero della Salute Ricerca Corrente-Linea 1 on emerging and re-emerging infectionsRegione Umbria SARS-CoV-2 epidemiological surveillance in urban wastewater (Regional Council resolution n. 1296, 16 December 2021)Unione Europea Wastewater Virome Analysis for Environmental Surveillance at hospital building level. Next Generation EU, Missione 4 - Componente 2 - LI 1.4 - CUP F53C24001620001
6 · The paper itself

Abstract

Wastewater surveillance has proven to be a cost-effective, non-invasive method for monitoring the spread and evolution of SARS-CoV-2, yet its value during today's low-incidence phase is still being defined. Between August 2023 and July 2024, 42 composite wastewater samples were collected in Perugia, Italy and analyzed using RT-qPCR and whole-genome sequencing to identify circulating SARS-CoV-2 lineages. In parallel, clinical samples (respiratory tract samples) were collected and analyzed, allowing for direct comparisons to confirm the robustness of the wastewater findings. The sewage viral loads ranged from 8.9 × 10

Indexed as

next-generation sequencingRT-qPCRSARS-CoV-2viral variantswastewater surveillance

Identifiers

PMID40566504
PMCPMC12193976

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.