Evidence map›Paper›PMID 40565545›Full record

ArticleGenes2025

Molecular Sexing in Owls (Aves, Strigiformes) and the Unique Genetic Structure of the Chromodomain Helicase DNA-Binding Protein 1 (CHD1) Gene on Chromosome W.

Mana Esaki, Kenki Momohara, Atsushi Haga, Maria Narahashi, Mu Mu Aung, Kaori Tokorozaki, Yuko Haraguchi, Kosuke Okuya, Isao Nishiumi, Manabu Onuma and 1 more

Abstract read
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In one paragraph

Article in Genes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Mana EsakiJoint Graduate School of Veterinary Science, Kagoshima University, Kagoshima 890-0065, Japan.ORCID 0009-0001-3440-2687
Kenki MomoharaDepartment of Pathogenetic and Preventive Veterinary Science, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan.
Atsushi HagaNational Institute for Environmental Studies, Tsukuba 305-8506, Japan.
Maria NarahashiGraduate School of Integrated Sciences for Global Society, Kyushu University, Fukuoka 819-0395, Japan.
Mu Mu AungForest Research Institute, Forest Department, Ministry of Natural Resources and Environmental Conservation, Yezin, Nay Pyi Taw P.O. Box 05282, Myanmar.
Kaori TokorozakiKagoshima Crane Conservancy, Izumi 899-0208, Japan.
Yuko HaraguchiKagoshima Crane Conservancy, Izumi 899-0208, Japan.
Kosuke OkuyaJoint Graduate School of Veterinary Science, Kagoshima University, Kagoshima 890-0065, Japan.ORCID 0000-0002-7987-9884
Isao NishiumiDepartment of Zoology, National Museum of Nature and Science Tokyo, Tsukuba 305-0005, Japan.ORCID 0000-0002-4627-3077
Manabu OnumaNational Institute for Environmental Studies, Tsukuba 305-8506, Japan.ORCID 0000-0001-8611-7506
Makoto OzawaJoint Graduate School of Veterinary Science, Kagoshima University, Kagoshima 890-0065, Japan.ORCID 0000-0002-7731-5321

Funding

Education and Research Center for Mathematical and Data Science, Kyushu University N/AJapan Science and Technology Agency JPMJSP213Japan Society for the Promotion of Science JSPS KAKENHI Grant Number 22K06379the National Museum of Nnature and Science, Tokyo Biological Inventory with special attention to Myanmarthe Takehiko Yamashina Grant Program (2023) N/A
6 · The paper itself

Abstract

backgroundThe accurate determination of bird sex is crucial in various biological fields, including ecology, behavioral research, and conservation. However, this task remains challenging in species in which males and females exhibit similar external morphologies, such as owls. Although polymerase chain reaction (PCR)-based molecular sexing techniques that target the chromodomain helicase DNA-binding protein 1 gene found on sex chromosomes Z (

methodsWe developed a new primer set and determined the nucleotide sequences-including the binding sites for the primers 2550F and 2718R-within both the

resultsSequencing analysis, conducted using a newly developed primer set that successfully amplified both Z- and W-derived

conclusionThese findings provide valuable insights for improving molecular sexing in owls.

Indexed as

DNA-Binding ProteinsSex ChromosomesSex Determination AnalysisStrigiformesAnimalsFemaleMaleDNA-Binding ProteinsCHD1 genemolecular sexingowl

Identifiers

PMID40565545
PMCPMC12191965

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