Evidence map›Paper›PMID 40565302›Full record

ArticleInternational journal of molecular sciences2025

Role of Extracellular Vesicles of Stem Cells from Human Exfoliated Deciduous Teeth in Osteogenesis.

Rio Shibata, Ryo Kunimatsu, Shota Ito, Tomohiro Ogasawara, Shintaro Ogashira, Ayaka Nakatani, Kodai Rikitake, Ayaka Odo, Akira Hirabae, Io Koyanagi and 5 more

Abstract read
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In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Rio ShibataDepartment of Orthodontics and Craniofacial Developmental Biology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, Japan.
Ryo KunimatsuDepartment of Orthodontics and Craniofacial Developmental Biology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, Japan.
Shota ItoDepartment of Orthodontics, Division of Oral Health and Development, Hiroshima University Hospital, Hiroshima 734-8553, Japan.ORCID 0000-0002-1907-9324
Tomohiro OgasawaraDepartment of Orthodontics, Division of Oral Health and Development, Hiroshima University Hospital, Hiroshima 734-8553, Japan.ORCID 0009-0001-2310-1248
Shintaro OgashiraDepartment of Orthodontics, Division of Oral Health and Development, Hiroshima University Hospital, Hiroshima 734-8553, Japan.
Ayaka NakataniDepartment of Orthodontics and Craniofacial Developmental Biology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, Japan.
Kodai RikitakeDepartment of Orthodontics, Division of Oral Health and Development, Hiroshima University Hospital, Hiroshima 734-8553, Japan.
Ayaka OdoDepartment of Orthodontics, Division of Oral Health and Development, Hiroshima University Hospital, Hiroshima 734-8553, Japan.
Akira HirabaeDepartment of Orthodontics and Craniofacial Developmental Biology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, Japan.
Io KoyanagiDepartment of Orthodontics and Craniofacial Developmental Biology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, Japan.
Takaharu AbeDepartment of Orthodontics and Craniofacial Developmental Biology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, Japan.ORCID 0000-0002-6764-0956
Tomoka HirakiDepartment of Orthodontics and Craniofacial Developmental Biology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, Japan.
Shuzo SakataDepartment of Orthodontics and Craniofacial Developmental Biology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, Japan.
Yuki YoshimiDepartment of Orthodontics, Division of Oral Health and Development, Hiroshima University Hospital, Hiroshima 734-8553, Japan.ORCID 0000-0001-7845-983X
Kotaro TanimotoDepartment of Orthodontics and Craniofacial Developmental Biology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, Japan.ORCID 0000-0003-4439-1579

Funding

Japan Society for the Promotion of Science 24K02653, 24K02654, 24K20059, 24K20082, 24K23554 22K10247 and 23K09415JST SPRING JPMJSP2132
6 · The paper itself

Abstract

The tissue regenerative potential of the liquid component of mesenchymal stem cells has gained significant attention. Stem cells from human exfoliated deciduous teeth-conditioned medium (SHED-CM), which is often extracted during orthodontic treatment, promotes bone regeneration. However, further investigation is warranted to determine which component of SHED-CM affects bone regeneration. Therefore, we focused on the extracellular vesicles contained in SHED-CM (SHED-EVs) and aimed to study their effects on osteoblasts. SHED-EVs were isolated using a pellet-down EV extraction kit and identified using transmission electron microscopy and NanoSight. SHED-EVs were added to human calvarial osteoblasts (HCOs), and cell proliferation and migration ability were examined with Incucyte

Indexed as

Extracellular VesiclesMesenchymal Stem CellsOsteogenesisStem CellsTooth, DeciduousAlkaline PhosphataseBone RegenerationCell DifferentiationCell MovementCell ProliferationCells, CulturedCulture Media, ConditionedHumansOsteoblastsAlkaline PhosphataseCulture Media, Conditionedbone regenerationculture supernatantEVsMSCsSHED

Identifiers

PMID40565302
PMCPMC12193389

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.