Evidence map›Paper›PMID 40564960›Full record

ArticleInternational journal of molecular sciences2025

Biphasic CAPA-IVM Improves Equine Oocyte Quality and Subsequent Embryo Development Without Inducing Genetic Aberrations.

Muhammad Fakhar-I-Adil, Daniel Angel-Velez, Emin Araftpoor, Qurratul Ain Amin, Mohamed Hedia, Marcel Bühler, Kris Gevaert, Björn Menten, Ann Van Soom, Susana Marina Chuva de Sousa Lopes and 4 more

Abstract read
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In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Muhammad Fakhar-I-AdilGhent-Fertility and Stem Cell Team (G-FaST), Department for Reproductive Medicine, Department of Human Structure and Repair, Ghent University Hospital, Corneel Heymanslaan 10, 9000 Ghent, Belgium.ORCID 0000-0003-0400-2700
Daniel Angel-VelezDepartment of Internal Medicine, Reproduction and Population Medicine, Ghent University, 9820 Merelbeke, Belgium.ORCID 0000-0001-8777-4065
Emin AraftpoorVIB-UGent Center for Medical Biotechnology, Vlaams Instituut voor Biotechnologie (VIB), 9052 Ghent, Belgium.ORCID 0000-0002-3584-7581
Qurratul Ain AminDepartment of Internal Medicine, Reproduction and Population Medicine, Ghent University, 9820 Merelbeke, Belgium.
Mohamed HediaDepartment of Internal Medicine, Reproduction and Population Medicine, Ghent University, 9820 Merelbeke, Belgium.
Marcel BühlerVIB-UGent Center for Medical Biotechnology, Vlaams Instituut voor Biotechnologie (VIB), 9052 Ghent, Belgium.ORCID 0000-0001-9608-1316
Kris GevaertVIB-UGent Center for Medical Biotechnology, Vlaams Instituut voor Biotechnologie (VIB), 9052 Ghent, Belgium.ORCID 0000-0002-4237-0283
Björn MentenCenter for Medical Genetics, Ghent University Hospital, 9000 Ghent, Belgium.
Ann Van SoomDepartment of Internal Medicine, Reproduction and Population Medicine, Ghent University, 9820 Merelbeke, Belgium.ORCID 0000-0001-5010-6311
Susana Marina Chuva de Sousa LopesDepartment of Anatomy and Embryology, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands.
Dominic StoopDepartment for Reproductive Medicine, Women's Clinic, Ghent University Hospital, Corneel Heymanslaan 10, 9000 Ghent, Belgium.
Chloë De RooDepartment for Reproductive Medicine, Women's Clinic, Ghent University Hospital, Corneel Heymanslaan 10, 9000 Ghent, Belgium.ORCID 0000-0002-6623-3283
Katrien SmitsDepartment of Internal Medicine, Reproduction and Population Medicine, Ghent University, 9820 Merelbeke, Belgium.ORCID 0000-0002-8205-3725
Björn HeindryckxGhent-Fertility and Stem Cell Team (G-FaST), Department for Reproductive Medicine, Department of Human Structure and Repair, Ghent University Hospital, Corneel Heymanslaan 10, 9000 Ghent, Belgium.

Funding

Bijzonder Onderzoeksfonds BOF.STG.2021.0042.01Bijzonder Onderzoeksfonds (BOF) 2018000504 (GOA030-18 BOF)Research Foundation - Flanders 1177425N
6 · The paper itself

Abstract

In vitro maturation (IVM) of oocytes retrieved from ovum pick-up (OPU) or ovarian tissue (OT) is a standard approach for patients with specific conditions where prior hormonal stimulation is contraindicated. However, the developmental competence of oocytes matured in vitro is still inferior to that of oocytes matured in vivo. Capacitation IVM (CAPA-IVM) includes an extra step of pre-maturation culture (PMC) with c-type natriuretic peptide (CNP) as a meiotic arrestor to better synchronize cytoplasmic and nuclear maturity in oocytes by allowing the cytoplasm additional time to acquire essential components critical for optimal competency. This study aims to evaluate the effect of CAPA-IVM on equine oocyte quality and developmental competence. Immature cumulus-oocyte complexes (COCs) were retrieved from slaughterhouse ovaries and matured in vitro either in CAPA-IVM (short 6 h, long 24 h pre-maturation) or standard IVM. Mature oocytes from each group were analyzed for calcium-releasing potential (

Indexed as

Embryonic DevelopmentIn Vitro Oocyte Maturation TechniquesOocytesAnimalsBlastocystChromosome AberrationsCumulus CellsFemaleHorsesNatriuretic Peptide, C-TypeSperm Injections, IntracytoplasmicNatriuretic Peptide, C-TypeCAPA-IVMequine embryosequine euploidyin vitro maturationoocyte proteomics

Identifiers

PMID40564960
PMCPMC12192595

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.