A Convenient Fluorogenic Detection Strategy for Phosphorothioate Modification of DNA Through Photocatalytic Oligonucleotide-Templated Reaction.

Nannan Jing, Yantian Qin, Xinli Fan, Qian Wang, Jing Wang, Fuping You, Xinjing Tang

Abstract read

In one paragraph

Article in Biomolecules, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

7 authors.

Nannan JingSchool of Basic Medical Sciences, Peking University, No. 38 Xueyuan Rd., Beijing 100191, China.

Yantian QinState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, No. 38 Xueyuan Rd., Beijing 100191, China.

Xinli FanState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, No. 38 Xueyuan Rd., Beijing 100191, China.

Qian WangState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, No. 38 Xueyuan Rd., Beijing 100191, China.

Jing WangState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, No. 38 Xueyuan Rd., Beijing 100191, China.ORCID 0000-0001-8369-7453

Fuping YouSchool of Basic Medical Sciences, Peking University, No. 38 Xueyuan Rd., Beijing 100191, China.

Xinjing TangState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, No. 38 Xueyuan Rd., Beijing 100191, China.

Funding

National Natural Science Foundation of China 22277003, 81821004, 22077005, 22107004, 22077008

6 · The paper itself

Abstract

DNA phosphorothioate (PT) modifications, characterized by the replacement of a non-bridging phosphate oxygen atom with a sulfur atom, are widely observed in bacterial genomes. Sensitive detection of phosphorothioate is crucial for elucidating their biological roles and functions. Herein, we developed an innovative method that leverages oligonucleotide-templated reactions (OTRs) and fluorogenic oligonucleotide probes. By optimizing temperature, probe sequence length, and the relative distance between PT position and the fluorophore probe, we achieved sensitive detection for DNA PT modifications through fluorogenic signal amplification, which provides an efficient and cost-effective approach for sensitive detection of phosphorothioate-modified DNA.

Indexed as

DNAFluorescent DyesOligonucleotidesPhosphatesPhosphorothioate OligonucleotidesCatalysisDNAFluorescent DyesOligonucleotidesPhosphatesPhosphorothioate Oligonucleotidesfluorescence probefluorogenic oligonucleotide probesoligonucleotide-templated reactionsphosphorothioate modificationssequence specificity

Identifiers

PMID40563395

DOI10.3390/biom15060752

PMCPMC12191357

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