Evidence map›Paper›PMID 40559454›Full record

ArticleMethods and protocols2025

Expression and Site-Specific Biotinylation of Human Cytosolic 5'-Nucleotidase 1A in

Nataliya Slater, Anuradha Sooda, Frank L Mastaglia, Sue Fletcher, Mark Watson, Merrilee Needham, Jerome D Coudert

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods and protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Nataliya SlaterPersonalised Medicine Center, Murdoch University, Murdoch, WA 6150, Australia.ORCID 0000-0002-9979-6074
Anuradha SoodaPersonalised Medicine Center, Murdoch University, Murdoch, WA 6150, Australia.ORCID 0000-0002-2576-3309
Frank L MastagliaPerron Institute for Neurological and Translational Science, University of Western Australia, Nedlands, WA 6009, Australia.
Sue FletcherPersonalised Medicine Center, Murdoch University, Murdoch, WA 6150, Australia.ORCID 0000-0002-8632-641X
Mark WatsonPersonalised Medicine Center, Murdoch University, Murdoch, WA 6150, Australia.ORCID 0000-0002-6438-9225
Merrilee NeedhamPersonalised Medicine Center, Murdoch University, Murdoch, WA 6150, Australia.ORCID 0000-0001-6591-1444
Jerome D CoudertPersonalised Medicine Center, Murdoch University, Murdoch, WA 6150, Australia.ORCID 0000-0001-6081-8494

Funding

Spinnaker Health Research Foundation 2024
6 · The paper itself

Abstract

Autoantibodies targeting cytosolic 5'-nucleotidase 1A (cN1A) are found in several autoimmune diseases, including inclusion body myositis (IBM), Sjögren's syndrome, and systemic lupus erythematosus. While they have diagnostic relevance for IBM, little is known about the autoreactive B cells that produce these antibodies. To address this, we developed a robust protocol for the expression and site-specific biotinylation of recombinant human cN1A in

Indexed as

autoantibodiesAviTagB cellscN1Acytosolic 5′-nucleotidase 1AIBM

Identifiers

PMID40559454
PMCPMC12195718

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.