Evidence map›Paper›PMID 40559041›Full record

ArticleInsects2025

Laboratory Test Indirectly Reveals the Unreliability of RNA-Dependent 16S rRNA Amplicon Sequences in Detecting the Gut Bacterial Diversity of

Miaomiao Li, Xin Cao, Linfeng Xu, Luyao Lin, Xiaoqing Wu, Susu Fan, Xinjian Zhang, Fangyuan Zhou

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Insects, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Miaomiao LiShandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), No. 28789 Jingshidong Road, Licheng District, Jinan 250103, China.
Xin CaoShandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), No. 28789 Jingshidong Road, Licheng District, Jinan 250103, China.
Linfeng XuShandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), No. 28789 Jingshidong Road, Licheng District, Jinan 250103, China.
Luyao LinShandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), No. 28789 Jingshidong Road, Licheng District, Jinan 250103, China.
Xiaoqing WuShandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), No. 28789 Jingshidong Road, Licheng District, Jinan 250103, China.
Susu FanShandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), No. 28789 Jingshidong Road, Licheng District, Jinan 250103, China.
Xinjian ZhangShandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), No. 28789 Jingshidong Road, Licheng District, Jinan 250103, China.ORCID 0000-0002-5839-6197
Fangyuan ZhouShandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), No. 28789 Jingshidong Road, Licheng District, Jinan 250103, China.

Funding

National Natural Science Foundation of China 32272530QLU Major innovation projects of education-industry integration pilot 2024ZDZX10Shandong Province Key Agricultural Project for Application Technology Innovation SDAIT-31-04Young Innovation Team Project of Higher Education in Shandong Province 2024KJI002
6 · The paper itself

Abstract

In insect-microbe symbiosis, understanding the diversity of associated bacteria is crucial. DNA-dependent sequence methods are widely used to assess microbial diversity in insects, but they cannot distinguish between live and dead microbes. In contrast, RNA-dependent sequencing can identify alive bacterial communities, making them more suitable for evaluating alive microbiota diversity. However, its practical reliability in insect-microbe symbiosis remains poorly validated. This study investigated larval gut bacteria diversity of

Indexed as

amplicon sequencegut microbeinsect–microbeonion maggotsymbiontsymbiosis

Identifiers

PMID40559041
PMCPMC12193304

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.