ArticleCells2025
Histology and Immunohistochemistry of Adipose Tissue: A Scoping Review on Staining Methods and Their Informative Value.
Article in Cells, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.
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Who cites it
11 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Intrapancreatic fat deposition shows distinct regional and compartmental associations with obesity and diabetes.iScience · 2026Article
- An overview of immunohistochemistry: Evidence and personal experience.The Journal of international medical research · 2026Review
- Adipogenesis and Stem Cell Activation in Facial Fat After Repeated Low-energy Continuous Monopolar Radiofrequency: Ultrasound, 3D Imaging, and Histological Study.Plastic and reconstructive surgery. Global open · 2026Article
- Effect of long-term use of very low-carbohydrate Ketogenic diet on the thyroid gland and adipose tissue of adult male albino rats (biochemical and histological study).Journal of molecular histology · 2026Article
- Effect of Granulocyte Colony-Stimulating Factor (G-CSF) and Botulinum Toxin Type A on Fat Graft Retention in a Rat Model.Aesthetic plastic surgery · 2026Article
- Subcutaneous White Adipose Tissue Derived from Female Mice Achieves Better Graft Outcomes Through the Involvement of Tregs.Aesthetic plastic surgery · 2026Article
- Exploring Adipose Tissue Complexity Through Omics Approaches: Implications for Health and Disease.Cells · 2026Review
- Strategic application of multilayer fat grafting in facial rejuvenation: a retrospective study.Frontiers in surgery · 2026Article
- Medial vs Central Upper Eyelid Fat: A Histological and Immunohistochemical Study of Structural and Vascular Features.Aesthetic surgery journal. Open forum · 2026Article
- Clinical Applications of Stromal Vascular Fraction and Stromal Vascular Matrix for Osteoarthritis: A Commentary.HSS journal : the musculoskeletal journal of Hospital for Special Surgery · 2025Article
Corrections and comments
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Authors and funding
14 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHistological and immunohistochemical analyses of adipose tissue are essential for evaluating the quality and functionality of lipoaspirates in regenerative medicine and fat grafting procedures. These methods provide insights into tissue viability, cellular subtypes, and extracellular matrix (ECM) composition-all factors influencing graft retention and clinical outcomes. PURPOSE: This scoping review aims to summarize the most commonly used staining methods and their applications in the histology and immunohistochemistry of adipose tissue. By exploring qualitative and quantitative markers, we seek to guide researchers in selecting the appropriate methodologies for addressing experimental and translational research.
methodsA systematic search was conducted using PubMed, Ovid, and the Cochrane Library databases from inception to 2024, employing Boolean operators ("lipoaspirate" OR "fat graft" OR "gauze rolling" OR "decantation" OR "coleman fat" OR "celt" OR "nanofat" OR "lipofilling" OR "human fat" AND "histol*"). Studies were included if they utilized histology or immunohistochemistry on undigested human adipose tissue or its derivatives. The inclusion criteria focused on peer-reviewed, English-language studies reporting quantitative and qualitative data on adipose tissue markers.
resultsOut of 166 studies analyzed, hematoxylin-eosin (H&E) was the most frequently employed histological stain (152 studies), followed by Masson Trichrome and Sudan III. Immunohistochemical markers such as CD31, CD34, and perilipin were extensively used to distinguish stromal vascular fraction (SVF) cells, adipocytes, and inflammatory processes. Studies employing semiquantitative scoring demonstrated enhanced comparability, particularly for fibrosis, necrosis, and oil cyst evaluation. Quantitative analyses focused on SVF cell density, mature adipocyte integrity, and ECM composition. Methodological inconsistencies, particularly in preparation protocols, were observed in 25 studies.
conclusionsThis review highlights the critical role of histological and immunohistochemical methods in adipose tissue research. H&E staining remains the cornerstone for general tissue evaluation in the clinical context, while specialized stains and immunohistochemical markers allow for detailed analyses of specific cellular and ECM components in experimental research. Standardizing preparation and evaluation protocols will enhance interstudy comparability and support advancements in adipose tissue-based therapies.
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