Evidence map›Paper›PMID 40557518›Full record

ArticleAnimal models and experimental medicine2025

Comparative analysis of two newly established Cre rat lines, NeuN-Cre and Thy1-Cre, for neurological research.

Keru Li, Chenyang Liu, Gefan Wan, Lei Zhang, Rui Fan, Xu Zhang, Yuanlong Su, Jiayue He, Ning Liu, Feifei Guan and 6 more

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Animal models and experimental medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

16 authors.

Keru LiState Key Laboratory of Respiratory Health and Multimorbidity, NHC Key Laboratory of Human Disease Comparative Medicine, Key Laboratory of Pathogen Infection Prevention and Control, Ministry of Education, National Human Diseases Animal Model Resource Center and National Center of Technology Innovation for Animal Model, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS), Peking Union Medical College (PUMC), Beijing, China.
Chenyang LiuState Key Laboratory of Respiratory Health and Multimorbidity, NHC Key Laboratory of Human Disease Comparative Medicine, Key Laboratory of Pathogen Infection Prevention and Control, Ministry of Education, National Human Diseases Animal Model Resource Center and National Center of Technology Innovation for Animal Model, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS), Peking Union Medical College (PUMC), Beijing, China.
Gefan WanState Key Laboratory of Respiratory Health and Multimorbidity, NHC Key Laboratory of Human Disease Comparative Medicine, Key Laboratory of Pathogen Infection Prevention and Control, Ministry of Education, National Human Diseases Animal Model Resource Center and National Center of Technology Innovation for Animal Model, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS), Peking Union Medical College (PUMC), Beijing, China.
Lei ZhangState Key Laboratory of Respiratory Health and Multimorbidity, NHC Key Laboratory of Human Disease Comparative Medicine, Key Laboratory of Pathogen Infection Prevention and Control, Ministry of Education, National Human Diseases Animal Model Resource Center and National Center of Technology Innovation for Animal Model, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS), Peking Union Medical College (PUMC), Beijing, China.
Rui FanState Key Laboratory of Vascular Homeostasis and Remodeling, Institute of Cardiovascular Sciences, School of Basic Medical Sciences, Peking University, Beijing, China.
Xu ZhangState Key Laboratory of Respiratory Health and Multimorbidity, NHC Key Laboratory of Human Disease Comparative Medicine, Key Laboratory of Pathogen Infection Prevention and Control, Ministry of Education, National Human Diseases Animal Model Resource Center and National Center of Technology Innovation for Animal Model, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS), Peking Union Medical College (PUMC), Beijing, China.
Yuanlong SuState Key Laboratory of Vascular Homeostasis and Remodeling, Institute of Cardiovascular Sciences, School of Basic Medical Sciences, Peking University, Beijing, China.
Jiayue HeState Key Laboratory of Respiratory Health and Multimorbidity, NHC Key Laboratory of Human Disease Comparative Medicine, Key Laboratory of Pathogen Infection Prevention and Control, Ministry of Education, National Human Diseases Animal Model Resource Center and National Center of Technology Innovation for Animal Model, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS), Peking Union Medical College (PUMC), Beijing, China.
Ning LiuState Key Laboratory of Respiratory Health and Multimorbidity, NHC Key Laboratory of Human Disease Comparative Medicine, Key Laboratory of Pathogen Infection Prevention and Control, Ministry of Education, National Human Diseases Animal Model Resource Center and National Center of Technology Innovation for Animal Model, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS), Peking Union Medical College (PUMC), Beijing, China.
Feifei GuanState Key Laboratory of Respiratory Health and Multimorbidity, NHC Key Laboratory of Human Disease Comparative Medicine, Key Laboratory of Pathogen Infection Prevention and Control, Ministry of Education, National Human Diseases Animal Model Resource Center and National Center of Technology Innovation for Animal Model, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS), Peking Union Medical College (PUMC), Beijing, China.
Wei DongState Key Laboratory of Respiratory Health and Multimorbidity, NHC Key Laboratory of Human Disease Comparative Medicine, Key Laboratory of Pathogen Infection Prevention and Control, Ministry of Education, National Human Diseases Animal Model Resource Center and National Center of Technology Innovation for Animal Model, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS), Peking Union Medical College (PUMC), Beijing, China.
Shan GaoState Key Laboratory of Respiratory Health and Multimorbidity, NHC Key Laboratory of Human Disease Comparative Medicine, Key Laboratory of Pathogen Infection Prevention and Control, Ministry of Education, National Human Diseases Animal Model Resource Center and National Center of Technology Innovation for Animal Model, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS), Peking Union Medical College (PUMC), Beijing, China.
Weining KongState Key Laboratory of Respiratory Health and Multimorbidity, NHC Key Laboratory of Human Disease Comparative Medicine, Key Laboratory of Pathogen Infection Prevention and Control, Ministry of Education, National Human Diseases Animal Model Resource Center and National Center of Technology Innovation for Animal Model, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS), Peking Union Medical College (PUMC), Beijing, China.
Xiaolong QiState Key Laboratory of Respiratory Health and Multimorbidity, NHC Key Laboratory of Human Disease Comparative Medicine, Key Laboratory of Pathogen Infection Prevention and Control, Ministry of Education, National Human Diseases Animal Model Resource Center and National Center of Technology Innovation for Animal Model, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS), Peking Union Medical College (PUMC), Beijing, China.
Xiaolin ZhangThe Second Affiliated Hospital of Chengdu Medical College, China National Nuclear Corporation 416 Hospital, Chengdu, China.
Yuanwu MaState Key Laboratory of Respiratory Health and Multimorbidity, NHC Key Laboratory of Human Disease Comparative Medicine, Key Laboratory of Pathogen Infection Prevention and Control, Ministry of Education, National Human Diseases Animal Model Resource Center and National Center of Technology Innovation for Animal Model, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS), Peking Union Medical College (PUMC), Beijing, China.ORCID 0000-0002-1882-1777

Funding

CAMS Innovation Fund for Medical Sciences 2021-I2M-1-024CAMS Innovation Fund for Medical Sciences 2021-I2M-1-034CAMS Innovation Fund for Medical Sciences 2023-I2M-2-001Fundamental Research Funds for the Central Universities 3332022040Fundamental Research Funds for the Central Universities 3332023164Haihe Laboratory of Cell Ecosystem Innovation Fund HH24KYZX0007Research Project of China Baoyuan Investment Co., Ltd Program CBYI202102State Key Laboratory Special Fund 2060204the Non-profit Central Research Institute Fund of the Chinese Academy of Medical Sciences 2023-PT180-01.Wellcome Trust 202411
6 · The paper itself

Abstract

backgroundThe Cre/loxP system is most popular in mice, but its application in rats has largely lagged far behind. The rat is vital laboratory animal, especially in toxicological and neurological studies. Generating genetic tools to manipulate neurons in rats could benefit neurological research.

methodsUsing the CRISPR/Cas9 system, we inserted a Cre cassette into endogenous Thy1 and NeuN loci. Thy1-Cre rats featured a downstream P2A-linked insertion, while NeuN-Cre was inserted at the transcriptional start site. The Cre activity was assessed by crossing with a Cre reporter (Rosa26

resultsWe generated two neuron-specific rats (Thy1-Cre and NeuN-Cre), which exhibited high neuron-specific Cre expression in brain and spinal cord with minor leakage in other tissues. Thy1-Cre showed minor leakage in spleen, lung and kidney while NeuN-Cre showed minor leakage in spleen and kidney. ND1

conclusionsWe successfully generated two neuron-specific NeuN-Cre and Thy1-Cre rats, and systemically analyzed their expression pattern.

Indexed as

IntegrasesNerve Tissue ProteinsNeuronsThy-1 AntigensAnimalsCRISPR-Cas SystemsMaleRatsRats, Sprague-DawleyRats, TransgenicCre recombinaseIntegrasesNerve Tissue ProteinsThy-1 AntigensCreND1NeuNneuron‐specificratThy1

Identifiers

PMID40557518
PMCPMC12464859

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.